玉米中基因激活蛋白KINASE 20调解了高温调节的口腔运动
Chuang Cheng1, Qiqi Wu2, Mei Wang1
1Key Laboratory of Plant Development and Environmental Adaption Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, China.
Plant physiology
|September 19, 2023
概括
一个新的玉米通路涉及ZmMKK9,ZmMPK20和ZmRIN2,在热应力下控制口腔的开放. 这一发现提高了对植物耐热性和水平衡机制的理解.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 高温可以导致危险的,不受控制的植物口腔开口.
- 了解调节口腔对热的反应的分子机制对于作物弹性至关重要.
研究的目的:
- 鉴定和描述参与调节在玉米中高温下口腔开口的基因.
- 通过口腔调节阐明控制热耐受性的分子途径.
主要方法:
- 乙基甲硫酸盐 (EMS) 突变发生,以创建玉米突变库.
- 在玉米 (Zea mays) 中进行基因鉴定,淘汰和过度表达研究.
- 蛋白相互作用测定,无处不在分析和病毒诱导的基因沉默 (VIGS).
- 在Arabidopsis thaliana中异质表达.
主要成果:
- 一个高温敏感 (hts) 突变被确定为ZmMPK20.20中的突变.
- ZmMPK20负面调节口腔的开口和水损失,增强热耐受性.
- 确定了ZmMKK9-ZmMPK20-ZmRIN2级联,其中ZmMKK9和ZmRIN2负面调节口腔开口.
结论:
- ZmMKK9-ZmMPK20-ZmRIN2信号级联是玉米中高温诱导的口腔开口的关键调节器.
- 这种途径平衡了水损失和叶子温度,从而提高了植物的耐热性.
- 针对这种途径可以提供提高作物对热应激的抵御能力的策略.
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