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在驼奶外体中测序和分析微RNA

Rinile Wu^1,2, Caifeng Yue^1,2, Yina Xie^1,2

¹1College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010011, China.

Acta veterinaria Hungarica

|September 19, 2023

概括

驼乳外基因组含有众多的微RNA (miRNA),包括新型的,这些基因向涉及各种生物过程和疾病的基因. 这些外体miRNAs可能解释驼牛奶的健康益处.

科学领域:

生物化学生物化学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

驼牛奶以其治疗性质而闻名.
外体是细胞间通信的关键媒介.
微RNAs (miRNAs) 是基因表达的关键调节者.

研究的目的:

在驼乳外体内识别和描述微RNA (miRNA).
为了研究这些外体miRNAs的功能作用.
探索驼牛奶miRNA及其健康益处之间的潜在联系.

主要方法:

驼牛奶外体的小RNA测序 (sRNA-seq).驼牛奶外体的小RNA测序.
生物信息分析用于miRNA识别和量化.
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径丰富分析.

主要成果:

确定了2,659个miRNAs,包括2,458个已知和201个新型miRNAs.
miR-148a和let-7i是已知的高度表达的miRNAs之一.
目标基因参与多细胞生物体的过程,代谢功能和细胞骨架等细胞组件.
针对的途径包括与阿尔茨海默病,非酒精性脂肪肝疾病和黄金杆菌感染有关的途径.

结论:

驼牛奶外基因组含有各种各样的miRNAs,具有重要的调节潜力.
这些外体miRNA的向基因涉及到各种病理状况.
这些发现表明,外体miRNAs有助于驼牛奶在疾病管理中的有益作用.

关键词:

驼的牛奶是驼的牛奶.外基因组是外基因组的组成部分.这就是miRNAs.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...