严重的肺炎和人类呼吸道上皮质的病原性损伤是由Coxsackievirus B4引起的
Jing Dai1, Duo Xu1, Chao Yang2,3
1State Key Laboratory of Respiratory Diseases, National Clinical Research Center for Respiratory Disease, Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Health, Guangzhou Medical University, Guangzhou, People's Republic of China.
考克萨基病毒B4 (CVB4) 肺炎菌株通过破坏呼吸道上皮屏障,导致严重的肺损伤. 这些菌株与手足口病菌株的不同之处在于它们的炎症和神经病毒性特征.
科学领域:
- 病毒学 病毒学
- 病变的发生和发病.
- 呼吸道感染 呼吸道感染
背景情况:
- 考克萨基病毒B4 (CVB4) 是一种严重的肠道病毒,死亡率高.
- 由于CVB4引起的重症肺炎的发病原因尚不清楚.
研究的目的:
- 为了研究导致严重肺炎的CVB4菌株的致病性.
- 为了比较肺炎衍生的CVB4与手足口病 (HFMD) 衍生的影响.
主要方法:
- 三个肺炎衍生的CVB4全长基因组的测序和注释4.
- 人类呼吸道上皮质 (HAE) 模型感染CVB4分离物.
- 病毒对HAE极性,紧密结节,乳毛和细胞因子分泌的影响的比较.
- 在人类神经母细胞瘤细胞中评估神经病毒性.
主要成果:
- 与HFMD衍生的CVB4 (GZ-HFM01) 相比,由肺炎衍生的CVB4 (GZ-R6) 导致HAE屏障的快速破坏,乳毛损失和过度缩小.
- 与HFMD衍生的CVB4相比,肺炎衍生的CVB4显示出HAE的基侧释放增加,并诱导早期细胞因子 (IL-6,IL-1β) 的水平降低.
- 从HFMD衍生的CVB4在SH-SY5Y细胞中表现出更大的神经毒性.
结论:
- 与HFMD衍生的菌株相比,肺炎衍生的CVB4在呼吸道中具有独特的致病机制.
- 这些发现提高了对CVB4诱导的肺炎的理解,有助于预防和治疗策略.
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