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Open Angle Glaucoma: Treatment

Open Angle Glaucoma: Treatment

In open-angle glaucoma, the iridocorneal angle remains open, but the trabecular meshwork becomes stiff, slowing down the outflow of aqueous humor. This causes a buildup of aqueous humor in the anterior chamber, leading to a sudden increase in intraocular pressure. The treatment for open-angle glaucoma focuses on reducing the elevated intraocular pressure by either decreasing the secretion of aqueous humor or increasing its outflow.
Drugs such as carbonic anhydrase inhibitors, α2- and...

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Updated: Jul 16, 2025

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

纳米纤维PCL为基础的人体带状网,用于水性湿气外流研究.

Maria Bikuna-Izagirre^1,2, Javier Aldazabal^1,2,3, Leire Extramiana⁴

¹University of Navarra, TECNUN School of Engineering, Manuel Lardizabal 13, 20018 San Sebastián, Spain.

ACS biomaterials science & engineering

|September 19, 2023

概括

研究人员使用聚卡普罗拉克脚架开发了一种新的体外模型,用于人类状状状网 (HTM) 和状状组织 (JCT). 这种模型有助于研究水性幽默的外流和人类尾管网的退化,用于玻璃眼癌研究.

关键词:

药物查对药物进行查.电力旋转是指电力旋转.玻璃眼 glaucoma 玻璃眼玻璃眼玻璃眼玻璃眼流出系统的流出系统.聚卡普罗拉克托尼是一种多重的产物.状网状网状网状网状网状网状网.

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Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

Translaminar Autonomous System Model for the Modulation of Intraocular and Intracranial Pressure in Human Donor Posterior Segments

Translaminar Autonomous System Model for the Modulation of Intraocular and Intracranial Pressure in Human Donor Posterior Segments

Published on: April 24, 2020

相关实验视频

Last Updated: Jul 16, 2025

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

Translaminar Autonomous System Model for the Modulation of Intraocular and Intracranial Pressure in Human Donor Posterior Segments

Translaminar Autonomous System Model for the Modulation of Intraocular and Intracranial Pressure in Human Donor Posterior Segments

Published on: April 24, 2020

科学领域:

生物医学工程生物医学工程
眼科医生眼科眼科
细胞生物学细胞生物学

背景情况:

主要的开角玻璃眼包括视神经退化,高眼内压 (IOP) 是一个关键的危险因素.
人体状管网 (HTM),特别是状管状组织 (JCT),调节水性幽默 (AH) 流出和IOP.
目前的体外JCT模型有限,阻碍了对AH外流和HTM退化的研究.

研究的目的:

开发一个功能性的 in vitro JCT 模型,使用聚烯酸 (PCL) 纳米纤维支架.
在工程JCT模型中调查形态学,细胞行为和药物反应.
为研究HTM细胞生物学和查影响尾外流的药物提供一个平台.

主要方法:

制造PCL纳米纤维支架用于JCT总结.
在脚手架上播种人类脊椎状细胞网.
使用SEM,qPCR和免疫化学对HTM标记物的表征.
构建一个压力敏感的 perfusion 系统来评估外流设施.
用德克萨米他和内塔苏迪尔测试药物反应.

主要成果:

PCL支架支持HTM细胞形态和功能,模仿了JCT.
工程 JCT 展示了特征性的 HTM 细胞标记物 (肌素,纤维素,原蛋白 IV) 和基因表达.
该模型展示了功能性外流特征和对IOP降低药物的反应性.
在体外成功地回顾了HTM细胞生物学和药物效应.

结论:

使用PCL纳米纤维支架成功开发了一种新的功能性体外JCT模型.
这个模型有效地模仿了人类JCT的关键方面,包括细胞形态,标记物表达和药物反应.
开发的模型是了解HTM细胞生物学,AH外流调节以及在青光眼研究中药物查的宝贵工具.