玉米转录因子CCT通过与Fra a1,E3酶WIPF2和auxin反应因子Aux/IAA8相互作用来调节干旱耐受性
Zhaoheng Zhang1, Jiayue Qu1, Min Lu1
1Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
Journal of experimental botany
|September 19, 2023
概括
玉米转录因子ZmCCT通过与关键蛋白相互作用和影响激素信号,调节植物对干旱的反应. 这提高了植物对非生物应激的耐受性.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 无生性压力因素显著影响植物生长和生产力.
- 玉米转录因子ZmCCT已知在光周期反应和疾病耐药性中的作用.
- 了解ZmCCT在应激反应中的功能对于作物改善至关重要.
研究的目的:
- 研究ZmCCT在植物对干旱反应中的作用.
- 阐明ZmCCT在非生物应激耐受性中的功能背后的分子机制.
主要方法:
- 酵母两杂交查以确定蛋白质相互作用.
- 在体外和体内测试蛋白质定位和活性.
- 分析ZmCCT过度表达的阿拉比多普西斯和玉米突变系.
- RNA测序和DNA亲和力净化测序.
主要成果:
- ZmCCT与ZmFra a1,ZmWIPF2和ZmAux/IAA8相互作用,局部化到不同的细胞区域.
- ZmCCT将ZmWIPF2招募到核中,从而增强其E3自我普遍化活动.
- ZmCCT影响了auxin和abscisic acid (ABA) 信号传递,可能作为ABA的负调节剂.
- ZmCCT促进了英多尔-3-酸生物合成,并调节了对干旱反应的基因.
结论:
- 在促进玉米非生物应激耐受性方面,ZmCCT发挥了多方面的作用.
- ZmCCT的功能包括蛋白质-蛋白质相互作用,激素信号调节和基因表达调节.
- 这项研究提供了ZmCCT对应激弹性贡献的详细分子机制.
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