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依赖的谷氨过氧化酶1调节了老鼠组织中的长酶3的转录
Ziqiao Sun1, Kun Wu1, Chenhan Feng1
1Department of Animal Science, Cornell University, Ithaca, NY, 14853, USA.
Free radical biology & medicine
|September 19, 2023
概括
谷氨过氧化酶1 (GPX1) 调节脂肪酸 (FA) 的合成. 过度表达GPX1通过抑制Elovl3基因转录来减少非常长链FA生物合成.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 营养科学 营养科学
背景情况:
- 在具有改变谷氨过氧化酶1 (GPX1) 表达的组织中观察到不受调节的脂质代谢.
- GPX1在调节组织脂肪酸 (FA) 生物合成方面发挥着作用,但潜在的机制需要进一步探索.
研究的目的:
- 研究GPX1在调节脂肪酸生物合成中的作用.
- 阐明GPX1影响FA合成中的基因表达的分子机制.
主要方法:
- 肝脏和脂肪组织的FA概况和基因表达在GPX1过度表达 (OE),缺陷 (KO) 和野生型 (WT) 小鼠中的比较分析.
- 在小鼠中使用二酸,乙素 (GPX1模仿剂) 和N-乙半氨酸 (NAC) 治疗,以评估对FA延长酶,脱酶和转录因子 (TF) 的影响.
- 路西法酶记者测定以确定GPX1模仿ebselen的Elovl3基因促进者的转录调节.
主要成果:
- 在GPX1 OE小鼠中,非常长链脂肪酸度降低 (C20:0,C22:0,C24:0),而KO小鼠则度增加.
- 与WT相比,Elovl3转录在OE小鼠中相互增加,在KO小鼠中相互减少.
- 埃布塞伦和NAC治疗降低了Elovl3转录,并且在Elovl3促进体中删除DBP结合部位减弱了埃布塞伦的抑制作用.
结论:
- 过度表达GPX1降低了非常长链脂肪酸生物合成的调节.
- 这种调节是通过对Elovl3基因促进者的转录抑制发生的.
- 通过调节关键的转录因子和Elovl3基因表达,GPX1影响FA生物合成.
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