相关实验视频
Updated: Jul 16, 2025

06:35
In vivo Evaluation of Mucociliary Clearance in Mice
Published on: December 18, 2020
4.4K
MAST4通过对Tctex-1的酸化促进了初级毛吸收
Kensuke Sakaji1, Sara Ebrahimiazar1,2, Yasuhiro Harigae1
1Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Life science alliance
|September 19, 2023
概括
微管相关的氨酸/氨酸激酶4 (MAST4) 通过控制酸化的Tctex-1定位来调节初级膜再吸收. MAST4是皮病的潜在治疗标.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 初级毛对于细胞功能至关重要,它们的失调会导致毛病.
- 化Tctex-1在底部的Thr94 (p-Tctex-1) 已知可以调节的再吸收.
- 纤毛动力学的缺陷与各种人类疾病有关.
研究的目的:
- 为了研究微管相关的氨酸/氨酸激酶家族4号成员 (MAST4) 在初级膜再吸收中的作用.
- 阐明MAST4调节纤毛再吸收的机制及其与Tctex-1的关系.
主要方法:
- 利用基于细胞的测试来研究初级膜动力学.
- 进行了MAST4敲击和过度表达实验.
- 使用生物化学方法研究了MAST4和Tctex-1之间的蛋白质-蛋白质相互作用.
- 在初级毛中分析了p-Tctex-1和MAST4的局部.
- 评估了MAST4在Cdc42激活和Rab5介导的内细胞分裂中的作用.
主要成果:
- MAST4定位在主上,并调节其再吸收.
- MAST4 枯竭阻断了血清诱导的纤维细胞再吸收,而其过度表达则加速了它.
- Tctex-1与MAST4的激酶域结合,其中特定的残留物R503和D504是关键的.
- MAST4对于p-Tctex-1的基定位,以及在再吸收过程中对Cdc42的激活和Rab5介导的内细胞分裂至关重要.
- 催化不活跃的MAST4突变体和MAST4倒置抑制纤维细胞吸收和p-Tctex-1定位.
结论:
- MAST4是一种新型激酶,可以控制初级膜再吸收.
- MAST4通过调节p-Tctex-1的纤毛基局部化来调节纤毛再吸收.
- MAST4 是一种潜在的治疗目标,用于与纤毛再吸收缺陷相关的纤毛病.
更多相关视频
相关概念视频
Microtubules in Signaling
1.7K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
1.7K
Mechanism of Ciliary Motion
3.7K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
3.7K
Cystic Fibrosis: Management
191
Cystic fibrosis (CF) is an autosomal recessive disorder that predominantly affects individuals of Northern European descent, occurring at a rate of 1 in 3500. It is caused by a genetic mutation in a gene on chromosome 7, most commonly the ΔF508 mutation, that codes for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. This results in thicker mucus secretions and obstruction pathologies in multiple organs, including the lungs and sinuses.
Sinus disease and chronic...
Sinus disease and chronic...
191
Cystic Fibrosis: Pathogenesis
275
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
275
Cell Motility through Blebbing
1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
1.9K
Cytoskeletal Coordination in Cell Migration
4.8K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.8K

