内源性细胞激活是成年斑马鱼的再生能力的基础
Stephanie L Tsai1, Steffany Villaseñor1, Rishita R Shah2
1Center for Regenerative Medicine, Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
NPJ Regenerative medicine
|September 19, 2023
概括
成年斑马鱼的纤维细胞可以再生受伤的肌,这与哺乳动物不同. 这项研究详细介绍了再生过程,为潜在的治疗策略提供了对愈合机制的见解.
科学领域:
- 比较生物学是比较生物学.
- 结节组织研究 结节组织研究
- 再生医学是一种再生医学.
背景情况:
- 肌对于力传输至关重要,但在哺乳动物中愈合不好,形成痕组织.
- 成年哺乳动物的肌细胞 (肌细胞) 不能有效地再生受损的肌.
- 斑马鱼为研究肌再生提供了一个独特的模型.
研究的目的:
- 为了研究成年斑马鱼肌再生的细胞机制.
- 定义斑马鱼肌修复的阶段和细胞基础.
- 为了确定参与斑马鱼肌再生的信号通路.
主要方法:
- 成年斑马鱼头骨面部肌的完整撕裂损伤模型.
- 多光子成像,谱系追踪和传输电子显微镜.
- 肌再生阶段和细胞贡献的分析.
主要成果:
- 斑马鱼的纤维细胞在受伤时激活,并完全再生肌,在6个月内恢复原蛋白超结构.
- 再生包括炎症,细胞增殖形成桥梁,以及矩阵重塑.
- 预先存在的纤维细胞是再生的主要来源.
- TGF-β信号传递对于细胞的招募和桥梁形成至关重要.
结论:
- 成年斑马鱼表现出了显著的肌再生能力.
- 细胞激活和扩散驱动斑马鱼肌的修复.
- 斑马鱼是了解肌愈合和开发治疗方法的宝贵模型.
相关概念视频
01:41Spermatogenesis
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
01:06Multipotency and Niche of Bulge Stem Cell
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...