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相关概念视频

Ion Exchange01:17

Ion Exchange

620
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
620
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

431
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
431
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

2.1K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
2.1K
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

276
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
276
Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

1.3K
Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
1.3K
Types Of Column Chromatography01:29

Types Of Column Chromatography

11.2K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
11.2K

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相关实验视频

Updated: Jul 16, 2025

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
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Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

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硫酸功能化的单立柱,用于高选择性的毛细血管电染色学分离.

Shuyu Dai1, Yun Guo1, Hongyan Mao1

  • 1College of Chemistry, Key Laboratory of Molecular Sensing and Harmful Substances Detection Technology, Zhengzhou University, Kexue Avenue 100, Zhengzhou, Henan, 450001, People's Republic of China.

Mikrochimica acta
|September 19, 2023
PubMed
概括

一种新的硫酸修饰的共价有机聚合物 (COP-SO3H) 被合成用于毛细血管电染色学. 这种新材料证明了各种分析物的高度选择性分离,显示了现实世界样本分析的巨大潜力.

关键词:
毛细血管电染色学 毛细血管电染色学单立体毛细血管是一种单质的毛细血管.纳米球形共价有机聚合物硫酸功能的功能化

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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
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科学领域:

  • 材料科学 材料科学 材料科学
  • 分析化学 分析化学
  • 聚合物化学 聚合物化学

背景情况:

  • 共价有机聚合物 (COP) 为分离科学提供可调节的特性.
  • 开发新的静止相对于提高色谱选择性和效率至关重要.

研究的目的:

  • 合成和表征一种新的乙烯功能化的COP (TAPT-DVA-COP) 和其硫酸修饰的版本 (COP-SO3H).
  • 评估这些COP作为毛细血管电染色学 (CEC) 中单体静止相的应用潜力.

主要方法:

  • 使用2,5-二维尼-1,4-甲 (DVA) 和2,4,6-三4-氨基) -1,3,5-三 (TAPT) 合成TAPT-DVA-COP.
  • 在合成后硫化TAPT-DVA-COP,生成COP-SO3H.
  • 使用物理兴奋剂制造CEC单体柱子.

主要成果:

  • 与TAPT-DVA-COP列相比,COP-SO3H单体列对各种分析物具有更高的选择性.
  • 静止阶段证明了单替代,基,基酸,核基和生物氨基的有效分离.
  • COP-SO3H显示出良好的可重现性和潜力,用于确定紧粉中的基酸盐和废水样本中的基酸盐.

结论:

  • 硫酸组和COP-SO3H中的芳香分子的协同作用提供了多种相互作用机制 (离子交换,疏水性,π-π,键).
  • COP-SO3H代表了高性能CEC分离的有希望的静止阶段.
  • 开发的材料显示在分析现实世界样本时的实际应用性.