水中暴露于铜和对Coregonus ussuriensis Bergensis的单一和联合毒性影响
Wei Gu1, Kai Ji1, Tianqing Huang1
1Cold Water Fish Industry Technology Innovation Strategic Alliance, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, PR China.
Ecotoxicology (London, England)
|September 19, 2023
概括
这项研究表明,铜和暴露会导致Coregonus ussuriensis的肝脏和损伤,氧化应激和免疫反应变化. 这些发现对于监测水生环境中的重金属污染至关重要.
科学领域:
- 水生毒理学 水生毒理学
- 环境科学 环境科学
- 鱼类生物学 鱼类生物学
背景情况:
- 铜 (Cu) 和 (Cd) 等重金属对水生生态系统构成重大威胁.
- 科雷戈纳斯 (Coregonus ussuriensis Berg) 是一种有价值的鱼类,但它对重金属污染的易感性尚未得到充分研究.
- 工业和农业的增长增加了C. ussuriensis息地的重金属污染风险.
研究的目的:
- 为了确定铜和对C. ussuriensis的急性毒性.
- 调查Cu和Cd单独或组合暴露对C. ussuriensis的组织病理学,氧化应激和免疫毒性影响.
- 为监测C. ussuriensis环境中的重金属污染提供数据.
主要方法:
- 进行了急性毒性测试,以确定96小时中位数致命度 (LC50) 和Cu和Cd的安全度.
- 在C. ussuriensis暴露于Cu,Cd或其组合的96小时内,其LC50值的20%.
- 组织病理学分析 (血素和欧染色),氧化应激标志物 (SOD,CAT,GSH,MDA) 和免疫基因表达 (IL-1β,TNF-α,MT) 在肝脏和组织中进行了评估.
主要成果:
- 96小时的LC50值为Cu的0.492 mg·L-1和Cd的1.548 mg·L-1,安全度分别为4.92 μg·L-1和15.48 μg·L-1.
- 暴露于Cu和Cd,单独或组合,诱导肝脏和组织的显著基因病理损伤 (真空化,亡,胀,增生),随着暴露时间的增加而恶化.
- 氧化应激标志物显示抗氧化酶活性 (SOD,CAT,GSH) 和MDA水平增加,而促炎性细胞因子表达 (IL-1β,TNF-α) 和金属氨酸 (MT) 基因表达被上调,其模式因暴露时间而异.
结论:
- 铜和暴露,单独或组合,诱导显著的组织病理损伤,氧化应激和免疫毒性在C. ussuriensis.
- 观察到的毒性影响是时间依赖的,在更长的暴露期内,严重程度增加和基因表达模式发生变化.
- 这项研究为评估重金属对C. ussuriensis的风险提供了关键的毒理学数据,并为水产养殖的环境监测策略提供了信息.
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