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在Aphis gossypii中,双链RNA调节的糖酶基因诱导死亡率和减少后代产量的淘汰

Gadigavarahalli Subbareddy Uma^1,2, Manjesh Saakre³, Jyoti Singh¹

¹Division of Entomology, Indian Agricultural Research Institute, New Delhi, India.

Functional & integrative genomics

|September 19, 2023

概括

使用双链RNA (dsRNA) 杀Aphis gossypii sucrase1 (Agsuc1) 基因,通过导致死亡率和减少后代产量,显著减少了虫种群,提供了潜在的生物杀虫剂策略.

科学领域:

昆虫学昆虫学是一门学科.
分子生物学分子生物学
生物技术是生物技术.

背景情况:

肠道糖酶对于像Aphis gossypii这样的吸水昆虫来说至关重要,以保持透平衡并利用花营养素.
破坏糖酶功能会影响虫的生存和繁殖.

研究的目的:

研究Aphis gossypii sucrase1 (Agsuc1) 基因沉默对虫死亡率和繁殖的影响.
评估针对Agsuc1的RNA干扰 (RNAi) 作为害虫控制策略的潜力.

主要方法:

合成的双链RNA (dsRNA) 通过体外和体内 (细菌表达) 方法准Agsuc1.
通过口服给Aphis gossypii的dsRNA,并评估死亡率和生殖率.

主要成果:

口服dsagsuc1导致A.gossypii的近50%的死亡率.
幸存的虫在后代产量方面出现了39-43%的减少.
细菌对dsRNA的表达被证明是一种具有成本效益的生产方法.

结论:

沉默Agsuc1基因是通过增加死亡率和减少繁殖来减少虫种群的有效策略.
细菌dsRNA的表达为开发生物杀虫剂提供了一种可行且具有成本效益的方法.
Agsuc1基因是基于RNAi的A. gossypii害虫控制的有希望的目标,可能导致转基因作物应用.

关键词:

一个叫Agsuc1的公司.亚菲斯的八piipii.细菌表达的dsrna是什么它们是RNAiRNAi.继承人是继承人的

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