生物杀伤剂triclosan作为Mytilus早期幼虫的潜在发育干扰剂
Teresa Balbi1,2, Angelica Miglioli3, Michele Montagna1
1Department of Earth, Environmental and Life Sciences-DISTAV, University of Genoa, Genoa, Italy.
Environmental science and pollution research international
|September 19, 2023
概括
特里克洛桑 (TCS) 扰乱了海洋无脊椎动物的发育,在环境相关的度下影响了幼虫的贝形成. 这种生物杀菌剂干扰生物矿化过程,对水生生态系统构成风险.
科学领域:
- 环境毒理学环境毒理学
- 海洋生物学 海洋生物学
- 发展生物学 发展生物学
背景情况:
- 三 (TCS) 是一种广泛使用的杀菌剂,在全球水生环境中普遍存在.
- TCS因其脂性,生物积累潜力和疑似内分泌干扰性质而闻名.
- 关于TCS对无脊椎动物发育的影响的数据有限,特别是在生命早期.
研究的目的:
- 评估三桑 (TCS) 对海洋双鱼Mytilus galloprovincialis幼虫早期发育的影响.
- 研究TCS毒性的潜在机制,重点关注基因表达和形成.
- 为了确定贝幼虫中TCS的最低观察效应度 (LOEC) 和EC50.
主要方法:
- 在广泛的TCS度 (0.001-1,000μg/L) 范围内,使用Mytilus galloprovincialis进行了48小时的幼虫测定.
- 分析了与矿化,内分泌信号和生物转化相关的基因转录在受精后24小时和48小时 (hpf).
- 利用色彩,偏光显微镜和扫描电子显微镜来评估的发育和CaCO3沉积.
主要成果:
- TCS显著影响了D-veliger的发展,LOEC为0.1μg/L,EC50为236.1μg/L.
- 暴露于TCS调节了参与矿化,内分泌信号发送,胺代谢和生物转换的基因的转录.
- 观察到CaCO3沉积的变化,没有外双断,外形成缺陷从0.1μg/L TCS.
结论:
- 在环境相关的度下,triclosan在早期的幼虫中起到发育干扰的作用.
- TCS主要干扰生物矿物化过程,导致显著的外形成缺陷.
- 这些发现强调了TCS对海洋无脊椎动物发展和生态系统健康的潜在风险.
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