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Updated: Jul 16, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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目标介导的滚动圆转录与CRISPR/Cas12a-Cas13a的合,用于同时检测HPV16和HPV18

Shiying Zhou1, Shuyu Zhu1, Zhen Huang2

  • 1Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, PR China.

Chemical communications (Cambridge, England)
|September 20, 2023
PubMed
概括

这项研究引入了一种新的异热测定方法,用于同时检测多个DNA点,如HPV16和HPV18. 该方法使用目标介导的滚动圆转录与CRISPR/Cas12a-Cas13a相结合,以实现高效和普遍的检测.

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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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