开发一种固相微提取方法,用于测定干中的尼古丁含量
Andreja Jurič1, Blanka Tariba Lovaković1, Maja Lazarus1
1Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000 Zagreb, Croatia. ajuric@imi.hr.
Analytical methods : advancing methods and applications
|September 20, 2023
概括
一种新的Headspace固体相微提取 (HS-SPME) 方法准确地测量了干中的尼古丁. 这种快速,无溶剂的技术有助于控制这些食品的消费者安全.
科学领域:
- 分析化学 分析化学
- 食品安全 食品安全
背景情况:
- 干燥野生中的高尼古丁含量经常被欧盟食品管制报告.
- 现有的分析方法需要大量的样品预处理,因此需要改进以进行有效的监测.
研究的目的:
- 开发和验证一个简化的头部空间固体相微提取 (HS-SPME) 方法,用于在干中量化尼古丁.
- 建立一个敏感,快速和成本效益的分析程序,用于日常的食品安全控制.
主要方法:
- 优化HS-SPME参数,包括纤维涂层,样本数量,温度,时间,脱落和盐添加.
- 气色谱-质谱 (GC-MS) 检测与矩阵匹配校准和同位素标记的内部标准校正相结合,用于定量分析.
- 对线性,检测和量化极限,精度和准确性进行方法验证.
主要成果:
- 最优化的方法表现出极好的线性 (r2 = 0.9994) 从0.025-1毫克/公斤-1.1.
- 达到低检测值 (0.005毫克/公斤-1) 和量化值 (0.017毫克/公斤-1),低于欧盟法规.
- 证实了高精度 (98.2-100.3%) 和精度 (<10% RSD),尼古丁含量确定在18个干燥的博莱图斯样本中.
结论:
- 开发的HS-SPME-GC-MS方法快速,简单,灵敏,无溶剂,并且具有成本效益.
- 这种经过验证的方法适用于例行监测和控制干中的尼古丁含量,确保消费者安全.
- 该程序有效地解决了在欧盟食品控制中改进分析方法的需求.
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