循环节的干扰降低了晚期孕期,非哺乳期奶牛的葡萄糖生成流
Linda M Beckett1, Shawn S Donkin1, Theresa Casey1
1Department of Animal Sciences, Purdue University, West Lafayette, IN 47907.
JDS communications
|September 20, 2023
概括
在怀孕晚期的奶牛中,循环节的干扰会减少肝脏的碳流来合成葡萄糖,从而影响葡萄糖平衡. 这种转变有利于能源生产而不是葡萄糖生成,可能会恶化代谢问题.
科学领域:
- 动物的新陈代谢
- 循环节生物学 循环节生物学
- 生殖生理学 生殖生理学
背景情况:
- 在牛的晚期怀孕期间的循环节障碍与低血糖和胰岛素抵抗等代谢障碍有关.
- 了解这些代谢变化背后的机制对于奶牛健康至关重要.
研究的目的:
- 为了调查是否改变了肝脏的碳流为葡萄糖生成驱动的代谢变化在葡萄糖平衡由于昼夜干扰.
- 为了将肝脏碳流的变化与奶牛的基因表达相关联.
主要方法:
- 孕期晚期的霍尔斯坦牛接受了控制光暗周期或相移 (PS) 周期.
- 肝脏活检用[U-13C]酸盐进行化,以追踪碳流量.
- 在肝脏样本上进行RNA测序,以分析基因表达.
主要成果:
- 阶段转移 (PS) 组显示了血清素与酸盐比率 (eSer:eAsp) 的丰富性降低,表明碳流向葡萄糖合成的减少.
- eSer:eAsp比率与 propionyl-CoA carboxylase (PCCB) 和酸脱酶子单元 D (SDHD) 基因表达有负相关性.
- 这些发现表明,酸碳主要用于PS奶牛的能量生产.
结论:
- 晚期孕期乳牛的循环节障碍通过改变肝脏碳流量来损害葡萄糖生成.
- 降低向葡萄糖合成的流量和增加酸的能量利用,有助于代谢障碍.
- 肝脏基因表达,特别是PCCB和SDHD,与这些改变的代谢途径有关.
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