在大学环境中重新设计SARS-CoV-2传染性呼吸道疾病监测计划
Kylie L King1,2, Rachel Ham1, Austin Smothers1,2
1Center for Innovative Medical Devices and Sensors (REDDI Lab), Clemson University, Clemson, SC, United States.
Frontiers in public health
|September 20, 2023
概括
一种新的基于唾液的多重检测试验可以准确检测SARS-CoV-2,流感A和B. 这种低成本,高通量测试为社区和教育环境提供了扩展的呼吸道疾病诊断.
科学领域:
- 分子诊断学 分子诊断
- 病毒学 病毒学
- 公共卫生 公共卫生
背景情况:
- 针对呼吸道感染的多重RT-qPCR测试通常依赖于鼻口腔扫描,存在成本和吞吐量限制.
- 之前的工作验证了针对SARS-CoV-2的低成本,高通量唾液基RT-qPCR测试,适用于全校范围的查.
研究的目的:
- 开发和验证多重呼吸道诊断面板,用于在唾液中同时检测SARS-CoV-2,A型流感和B型流感.
- 评估测试的准确性,对SARS-CoV-2检测的影响,以及唾液中A型流感的稳定性.
主要方法:
- 在单个唾液样本中开发多重RT-qPCR试验以检测SARS-CoV-2,A型流感和B型流感.
- 临床验证比较测试结果与已确定的诊断方法.
- 使用合成RNA对照的模拟共感染的测试和评估室温下唾液中的流感A稳定性.
主要成果:
- 多重试验在检测流感A时达到93.5%的准确性 (43/46一致结果),而不会影响SARS-CoV-2的检测.
- 该试验成功检测了不同病毒度的模拟共感染.
- 在室温下,甲型流感在唾液中的稳定性被证实在室温下持续长达5天.
结论:
- 一种新的,具有成本效益的基于唾液的多重RT-qPCR测定方法可以同时检测SARS-CoV-2,A型流感和B型流感.
- 这种测试为社区和教育环境中扩大呼吸道疾病诊断提供了可行的高通量解决方案.
- 重新利用现有的实验室基础设施可以促进这种可访问的诊断工具的广泛实施.
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