miR-335-5p通过直接向TMJ OA中的SP1来抑制内分泌骨化,从而抑制内分泌骨化
Simo Xia1, Jiong Zhao1, Dahe Zhang1
1Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Oral diseases
|September 20, 2023
概括
微RNA-335-5p (miR-335-5p) 抑制了关节骨关节炎的内分泌骨化. 抑制miR-335-5p可能为这种情况提供治疗策略.
科学领域:
- 生物医学研究的研究.
- 分子生物学分子生物学
- 骨关节炎的研究研究.
背景情况:
- 关节骨关节炎 (TMJOA) 涉及到受损的内分泌骨化和状骨退化.
- 微RNA-335-5p (miR-335-5p) 在TMJOA内分泌骨化中的特定作用尚不清楚.
研究的目的:
- 在TMJOA的背景下,研究miR-335-5p在内分泌骨化中的作用.
- 探索针对TMJOA治疗的miR-335-5p的潜力.
主要方法:
- 在骨关节炎软骨中寻找上调的microRNA和目标基因.
- 评估了miR-335-5p对内分泌骨化在体外使用冠状细胞 (C28/I2) 与模仿剂和抑制剂的影响.
- 在老鼠TMJOA模型中通过对agomiR-335.5的关节内注射评估miR-335的体内调节.
主要成果:
- miR-335-5p水平受到上调,而内分泌骨化基因在TMJOA状软骨中受到下调.
- 在体内,miR-335-5p的使用抑制了内分泌骨化.
- 在体外,AgomiR-335抑制了矩阵矿化,miR-335-5p针对SP1,促进TGF-β/Smad信号传递,从而负面调节胆细胞活动.
结论:
- miR-335-5p显著抑制了内分泌骨化.
- 抑制miR-335-5p为关节骨关节炎提供了潜在的治疗途径.
相关概念视频
01:24Bone Formation by Endochondral Ossification
4.6K
Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...
4.6K
01:16TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
01:29Bone Formation by Intramembranous Ossification
6.3K
Intramembranous ossification is one of the two processes involved in the development of bones within an embryo. The flat bones of the face, most of the cranial bones, and the clavicles are formed via this process. During intramembranous ossification, the bones develop directly from sheets of undifferentiated mesenchymal connective tissue.
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into ...
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into ...
6.3K
01:31Osteoclasts in Bone Remodeling
3.0K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.0K
01:40Bone Remodeling
38.4K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.4K