AT Hook-Like 10酸化决定了核糖体RNA处理6-like 1 (RRP6L1) 染色体协会和在水应激期间的生长抑制
Min May Wong1, Xin-Jie Huang1, Yu-Chiuan Bau1
1Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
Plant, cell & environment
|September 20, 2023
概括
在干旱期间,AT Hook-Like 10 (AHL10) 的酸化控制了植物的生长. AHL10与调节表观遗传变化的蛋白质RRP6L1相互作用,以抑制水应激下的生长.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物应激生理学 植物应激生理学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- AT Hook-Like 10 (AHL10) 是一种植物特有的DNA结合蛋白,对生长调节至关重要.
- 化AHL10对于抑制干旱压力下的生长至关重要 (低水位潜力,pcw).
研究的目的:
- 阐明AHL10酸化调解干旱反应的机制.
- 确定与AHL10相互作用的蛋白质,并描述它们在干旱耐受性中的作用.
主要方法:
- 蛋白质与蛋白质相互作用研究以确定AHL10相互作用体.
- 在低水潜力下分析突变表型 (ahl10,rrp6l1,ahl10-1rrp6l1-2).
- 染色体沉试验用于评估RRP6L1染色体协会.
- 转录组分析以评估基因表达变化.
主要成果:
- 参与表观遗传调节的RRP6L1与AHL10相互作用.
- 缺乏AHL10或RRP6L1的突变体在干旱期间表现出增强的生长维护.
- 在干旱下,RRP6L1染色体协会取决于AHL10酸化.
- AHL10和RRP6L1协调调节压力和发育基因.
- 在生长调节方面,AHL10和同类AHL13具有不同的,非冗余的作用.
结论:
- 通过压力信号通路的AHL10酸化控制RRP6L1染色体协会.
- 在干旱期间,美里系统细胞中的AHL10-RRP6L1相互作用降低了干旱期间的生长.
- AHL10和AHL13在管理压力下的植物生长方面具有明显的功能,可能是通过异构复合体的形成.
相关概念视频
01:56Riboswitches
8.2K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.2K
01:23Stringent Response in E. coli
31
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
31
01:23Types of RNA
63.9K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.9K
01:30Other Stress Responses in Bacteria
33
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
33
01:23Transcriptional Regulation: Riboswitches
61
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
61
01:29Translational Regulation
42
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
42