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基于朱洛利丁的小分子探针用于活细胞中RNA的光成像

Iswar Chandra Mondal¹, Priya Rawat², Maksym Galkin³

¹School of Chemical Sciences, Indian Institute of Technology Mandi, H.P-175005, India.

Organic & biomolecular chemistry

|September 20, 2023

概括

研究人员开发了新的光探针 (BTZ-JLD和SEZ-JLD) 用于成像细胞内RNA. 这些探测器具有高亮度和选择性,能够清晰地可视化活细胞内的RNA,特别是在细胞核中.

科学领域:

化学生物学化学生物学
分子成像学分子成像学
有机化学有机化学

背景情况:

细胞内RNA成像是至关重要的,但由于合适的有机小分子探针的稀缺性而受到限制,特别是那些具有高量子产量和长波长特性的探针.
现有的探测器往往缺乏有效的细胞RNA可视化所需的亮度和特异性.

研究的目的:

设计,合成和评估新型阴离子朱洛利丁-阿结合物作为细胞内RNA的开启光探针.
为探测器与细胞内RNA相互作用和成像建立结构效率关系.
评估这些探针对于生物研究,特别是细胞成像应用的适用性.

主要方法:

三种阴阳性朱洛利丁-阿佐联合体的合成:OX-JLD,BTZ-JLD和SEZ-JLD.
光物理特征以确定量子产量,亮度和光谱属性.
在活细胞中使用细胞消化试验和光显微镜评估RNA相互作用和选择性.
研究影响探头性能的结构-活动关系.

主要成果:

探测器在与RNA结合时表现出具有高量子产量和亮度的"开启"光,这归因于受限的分子内旋转.
BTZ-JLD和SEZ-JLD展示了对RNA相互作用和成像的有希望的光物理特性和光学反应.
这些探测器成功地可视化了细胞内RNA,在生理条件下,活细胞中核细胞显著染色.
细胞消化试验证实了BTZ-JLD和SEZ-JLD显著的RNA选择性.
SEZ-JLD显示了细胞粘度的最小干扰,证实了其作为深红色RNA成像的细胞膜透探针的实用性.

结论:

阴离子朱洛利丁-阿佐联合体,特别是BTZ-JLD和SEZ-JLD,是细胞内RNA的有效开启光探针.
这些探测器提供高亮度,良好的RNA选择性,并使活细胞中RNA的深红色成像成为可能,包括细胞核.
开发的探针代表了推进细胞RNA研究和诊断的宝贵工具.

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