介酶体 stromal 细胞分泌分子提高了人类小岛的功能生存
Tzu-Wen Hong1, Sara Caxaria2, Lydia F Daniels Gatward1
1Department of Diabetes, School of Cardiovascular and Metabolic Medicine and Sciences, King's College London, London, UK.
Diabetic medicine : a journal of the British Diabetic Association
|September 20, 2023
概括
用尾酒预处理人体小岛的介质细胞 (MSC) 衍生的可以提高其在移植后的生存和功能. 这改善了接受者的葡萄糖控制,为细胞治疗提供了一个有前途的策略.
科学领域:
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 1型糖尿病 (T1D) 治疗依赖于人体小岛移植,但移植后的贝塔细胞损失限制了移植的存活率.
- 在临床前模型中,介酶体 stromal 细胞 (MSC) 和它们分泌的因子显示出改善岛屿移植结果的潜力.
研究的目的:
- 在T1D小鼠模型中评估MSC分泌的特定尾酒在增强人类小岛移植功能生存方面的疗效.
- 评估这种合剂对人类小岛的葡萄糖刺激胰岛素分泌 (GSIS) 和亡的影响.
主要方法:
- 人类小岛被处理为附录A1 (ANXA1),树皮细胞衍生因子-1 (SDF-1/CXCL12) 和补充成分C3 (C3a) 的尾酒.
- 试验室评估包括GSIS和caspase 3/7活动来测量亡.
- 在体内,使用T1D小鼠模型评估疗效,在囊下移植人类小岛,随后进行STZ诱导的β细胞切除.
主要成果:
- 在实验室中,MSC衍生的合剂显著增加了GSIS,并减少了人类小岛的亡.
- 用尾酒预处理的移植的人类小岛,在小鼠中维持了高达28天的正常血糖.
- 在接受预处理小岛的人群中观察到改善的葡萄糖耐受性,这表明活体内功能生存率提高.
结论:
- 用定义的MSC衍生的尾酒预处理人类小岛,可以改善小岛质量和1型糖尿病移植的功能性存活率.
- 这种方法代表了一种潜在的策略,以提高1型糖尿病的基于细胞的疗法的疗效.
相关概念视频
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
1.3K
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
Insulin and C-peptide are...
1.3K
Mesenchymal Stem Cells
4.8K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.8K
Insulin Secretory Vesicles
5.0K
Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
5.0K
Tissue Renewal without Stem Cells
1.7K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
1.7K


