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在Arabidopsis蛋白质组中由甲基原体诱导的变化涉及PATELLIN 4在氧化应激反应中的作用

Pavol Melicher¹, Petr Dvořák¹, Jan Řehák¹

¹Department of Biotechnology, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic.

Journal of experimental botany

|September 20, 2023

概括

反应性氧物种会在植物中引起氧化应激,改变蛋白质水平. 缺少铁氧化二甲基酶1 (FSD1) 的突变体显示出明显的蛋白质变化,特别是在叶绿体和口腔闭合调节中.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

光合作用在叶绿体中产生反应性氧物种 (ROS),可能导致氧化应激.
氧化应激会影响蛋白质的合成,降解和复杂的组装/拆卸.
铁超氧化物二甲酸酶1 (FSD1) 在减轻植物中氧化应激的作用.

研究的目的:

为了研究甲基维奥基因诱导的野生类型阿拉比多普西斯和fsd1突变体中的蛋白质变化.
识别受氧化应激和FSD1缺乏影响的蛋白质和通路.
探索PATELLIN 4在植物氧化应激反应中的作用.

主要方法:

枪式蛋白质组学被用来分析蛋白质丰度的变化.
在野生型阿拉比多普西斯和fsd1-1/fsd1-2淘汰突变之间进行了比较分析.
反向遗传学 (淘汰赛和补充线) 用于研究PATELLIN 4的功能.

主要成果:

甲基生物治疗改变了所有线的叶绿体和细胞质中的蛋白质水平.
与野生类型相比,fsd1突变体表现出代谢蛋白,叶绿体伴侣和有机细胞蛋白比率的显著变化.
在参与光系II分解的蛋白质中观察到特定的变化,以及PATELLIN 4丰度的减少.
帕特林4缺乏影响了口腔关闭,这表明在氧化应激反应中发挥了作用.

结论:

缺少FSD1导致明显的蛋白质变化,以应对氧化应激.
在氧化应激过程中,PATELLIN 4参与调节口腔关闭.
蛋白质组学提供了关于植物对蛋白质水平上的氧化损伤的反应的见解.

关键词:

阿拉比多普西斯 (Arabidopsis) 是一种植物.铁超氧化物二甲酶 1 铁超氧化物二甲酶帕特林 4 帕特林类塑料是一种塑料.甲基维奥基因的使用方法氧化应激是一种氧化应激.光合作用光合作用.血膜是一种等离子体膜.蛋白质组学蛋白质组学

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