工程菌细胞工厂用于光合作用生产果糖
Jiahui Sun1,2,3,4, Zhichao Zhang1,2,3, Shanshan Zhang1,2,3,4
1Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China.
ACS synthetic biology
|September 20, 2023
概括
菌可以被设计为直接的,使用二氧化碳和太阳能的一步性果糖生产. 这种光合作用生物制造方法实现了超过6g/L的果糖,为传统方法提供了有效的替代方案.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 光合成生物制造 光合成生物制造
背景情况:
- 果糖是一种具有广泛工业应用的关键单糖.
- 目前的果糖生产依赖于使用植物生物质的多步骤工艺.
- 菌代谢工程提供了一个可持续的,单步替代方案.
研究的目的:
- 为了设计蓝藻细菌作为有效的细胞工厂,直接生产果糖.
- 用二氧化碳和太阳能优化果糖的光合作用生产.
- 为了证明蓝藻细菌生物制造果糖的可行性.
主要方法:
- 通过敲除果糖酶来对 *Synechococcus elongatus* PCC 7942进行工程设计.
- 鉴定和利用原生糖合成途径用于生产果糖.
- 优化代谢,生理和种植参数的优化.
- 对 *Synechococcus* sp. 的工程范式的调整 在PCC 7002上.
主要成果:
- 经过工程设计的 *Synechococcus elongatus* PCC 7942 产生了 3.9 g/L 的果糖.
- 经过工程改造的 *Synechococcus* sp. 在 在PCC 7002中达到6g/L以上的果糖.
- 果糖从培养中提取了86%的纯度.
结论:
- 建立了一个成功的范式,用于为光合作用果糖生产的工程蓝藻细菌.
- 菌光合作用生物制造是生产果糖的可行和有效途径.
- 这种方法为传统的果糖制造提供了一个可持续的替代方案.
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