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用Pseudomonas putida注射的生物炭减轻了其抑制作用,减轻了氨酸污染土壤中生物降解途径的抑制作用

Jinfeng Lu¹, Yuexian Liu², Ruili Zhang²

¹College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100049, China.

Journal of hazardous materials

|September 20, 2023

概括

生物炭修订可以抑制多环芳 (PAH) 降解,但用特定细菌 (如Pseudomonas putida) 进行注射可以减轻这种效应. 这项研究揭示了生物炭背后的机制.

科学领域:

环境科学环境科学
微生物学微生物学
生物化学生化学

背景情况:

生物炭修改对土壤多环芳 (PAH) 生物降解的影响是有争议的,具有促进和抑制的潜力.
了解微生物和代谢对生物炭的反应对于优化其在污染土壤修复中的应用至关重要.

研究的目的:

阐明生物炭修改影响土壤微生物降解 (PHE) 的机制.
研究细菌注射在减轻生物炭对PAH生物降解的抑制作用中的作用.

主要方法:

代谢分析分析,以分析不同处理的土壤中的代谢变化.
超基因组分析以评估微生物社区结构和功能基因的变化.
用生物炭修正 (BB) 和用Pseudomonas putida (BP) 接种的生物炭应用于被酸盐污染的土壤.

主要成果:

生物炭修正案 (BB) 抑制了土著土壤细菌的PHE生物降解,而BP处理减轻了这种抑制.
代谢学家确定了9-phenanthrol作为BB治疗影响的关键差异性代谢物.
甲基因组学揭示了生物炭减少的细胞色素P450单氧化酶 (CYP116) 丰富性,影响PHE降解途径.

结论:

生物炭修正可以通过改变微生物代谢和减少关键功能基因来抑制PAH生物降解.
用特定的降解细菌注射生物炭,例如Pseudomonas putida,可以克服生物炭的抑制作用.
这项研究为PAH修复过程中生物炭-土壤-微生物相互作用提供了机制性的见解.

关键词:

用细菌接种的生物炭.代谢途径的代谢途径在P450单氧基因酶中.南的生物降解方法土壤土著细菌联盟土壤土著细菌联盟

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