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长期化物暴露对小鼠天生的免疫力,肠道机械和免疫屏障的影响

Yan Wang¹, Jing Xu², Hang Chen³

¹Institute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, Nanchong 637000, Sichuan, China.

Research in veterinary science

|September 20, 2023

概括

长期过多的化物摄入会损害小鼠的肠道屏障. 这项研究表明,化物暴露会损害先天免疫力,机械完整性和免疫功能,增加肠道透性.

科学领域:

免疫学免疫学免疫学
毒理学毒理学毒理学
胃肠病学胃肠病学

背景情况:

化物是一种常见的环境污染物.
了解化物对胃肠道系统的影响对于公共卫生至关重要.

研究的目的:

研究慢性化物暴露对小鼠天生的免疫力和肠道屏障功能的影响.
评估由于化物导致的肠道结构和组织损伤.

主要方法:

在120天的时间里,C57BL/6小鼠被暴露在不同度的化 (0.50,100,125 mg/L) 中.
组织学分析,ELISA和MTT色度测试用于评估肠道形态,屏障蛋白,免疫细胞活性和炎症标志物.

主要成果:

化物暴露导致肠损伤,杯状细胞减少,并减少紧结蛋白 (ZO-1,Claudin-1,Occludin) 的表达.
肠道透性的关键指标,如二胺氧化酶 (DAO),内毒素 (ET) 和D-乳酸 (D-LA),随着化物度的增加而增加.
天生的免疫功能,包括自然杀手 (NK) 细胞活性,中性粒细胞和红细胞参数,以及淋巴细胞计数,被化物抑制.

结论:

慢性过量摄入化物会损害肠道的机械和免疫屏障.
长期化物暴露导致肠道透率增加和随后的损伤.

关键词:

化物的化物.免疫障碍物免疫障碍物这是天生的免疫力.肠道肠道肠道机械屏障是一种机械屏障.

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