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精子传递的 lncRNA loc100847420 改善了早期牛胚胎的发育

Heqiang Li¹, Zheng Wang¹, Baobao Zhao¹

¹Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi 712100, PR China; College of Veterinary Medicine, Northwest A&F University, Ministry of Agriculture and Rural Affairs, Northwest A&∼F University, Yangling, Shaanxi, 712100, PR China.

Animal reproduction science

|September 20, 2023

概括

精子传递的长非编码RNA (lncRNAs) 对牛胚胎发育至关重要. 在受精期间转移的lncRNA loc100847420增强了早期胚胎发育和胚胎细胞发育率.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
发展生物学发展生物学

背景情况:

长非编码RNAs (lncRNAs) 作为竞争的内源RNAs (ceRNAs) 起作用,在牛胚胎发育中至关重要.
精子携带的lncRNAs在早期牛胚胎发育中的特定作用在很大程度上仍未被描述.

研究的目的:

调查精子传递 lncRNAs 在牛胚胎植入前发育中的调控作用.
为了识别影响早期胚胎发育的精子传播的特定 lncRNAs.

主要方法:

使用高通量测序来识别牛精子中的lncRNA.
使用定量实时PCR (qPCR) 来验证lncRNA的丰富和表达.
功能分析涉及将野生型和突变型的loc100847420注入代体胚胎.

主要成果:

发现 lncRNA loc100847420 在牛精子中具有高度丰富性,并在受精期间传递给卵细胞.
注射野生类型的loc100847420显著改善了胚芽细胞率,总细胞数,内细胞质量 (ICM) 细胞计数和热囊细胞 (TE) 细胞计数.
野生类型和突变性loc100847420注射之间没有观察到对切割率的显著影响.

结论:

精子传递的 lncRNA loc100847420 在增强早期牛胚胎的发育潜力方面发挥着积极作用.
这一发现强调了父系因素,特别是lncRNAs在调节胚胎发育中的重要性.

关键词:

牛类牛类牛类牛类牛类牛早期胚胎发育的早期发展这是一个LncRNARNA.通过精子传播的精子

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...