捐赠白细胞差异是全血基因表达模式的最大决定因素
Grant C O'Connell1, Jing Wang1, Christine Smothers2
1Molecular Biomarker Core, Case Western Reserve University, Cleveland, OH, USA; School of Nursing, Case Western Reserve University, Cleveland, OH, USA.
Genomics
|September 20, 2023
概括
全血中的基因表达主要取决于白细胞数量,而不是其他生物因素. 对细胞计数变异的计算对于准确解释转录基因数据至关重要.
科学领域:
- 基因组学就是基因组学.
- 免疫学 免疫学 免疫学
- 文字转录学 (Transcriptomics) 是一个学科.
背景情况:
- 细胞组成在大量组织转录学中显著影响基因表达模式.
- 许多全血转录学研究忽视了样本间的细胞性差异,假设观察到的基因表达变异源于真正的转录差异.
研究的目的:
- 量化白细胞计数与其他生物因素对全血转录水平变异的贡献.
- 评估细胞性对使用全血基因表达研究的混效应.
主要方法:
- 招募了138名人类捐赠者的队列.
- 使用了下一代测序和流细胞计.
- 白细胞计数 (中性粒细胞,淋巴细胞) 和其他因素 (年龄,性别,种族,种族,疾病状态) 的量化变异.
主要成果:
- 单单白细胞计数 (中性粒细胞和淋巴细胞) 解释了高达75%的全血转录水平对蛋白质编码基因的差异.
- 其他生物因素,如年龄,性别,种族,种族和常见疾病对转录水平的影响很小.
- 细胞计数异质性是全血基因表达差异的主要驱动因素.
结论:
- 大多数全血实验中的基因表达差异是由潜在的白细胞计数变化驱动的.
- 准确的全血转录基因数据的生物学解释需要考虑细胞计数异质性.
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