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Updated: Jul 16, 2025

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
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Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

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无细胞色素免疫沉用于检测心脏移植中的分子通路

Moon Kyoo Jang1, Tovah E Markowitz2, Temesgen E Andargie1,3

  • 1Genomic Research Alliance for Transplantation (GRAfT) and Laboratory of Applied Precision Omics, National Heart, Lung, and Blood Institute (NHLBI), NIH, Bethesda, MD, USA.

Life science alliance
|September 20, 2023
PubMed
概括

无等离子细胞DNA染色质免疫沉降测序 (cfChIP-seq) 为监测心脏移植患者提供了一种非侵入性方法. 这种技术可以检测分子通路和组织损伤,从而有可能在没有侵入性活检的情况下改善患者的治疗.

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相关概念视频

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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