通过Trypanosoma brucei的转化控制DRBD18有助于维持前循环状态
Martin Ciganda1, José Sotelo-Silveira2, Ashutosh P Dubey1
1Department of Microbiology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York 14203, USA.
RNA结合蛋白DRBD18通过控制翻译来调节Trypanosoma brucei中的基因表达. 削弱DRBD18会改变蛋白质的产生,影响寄生虫的发展和宿主适应.
科学领域:
- 分子寄生虫学 分子寄生虫学
- 基因规则 基因规则
- 在RNA生物学,RNA生物学.
背景情况:
- 虫 (Trypanosoma brucei) 通过改变其蛋白质格局来适应不同的宿主环境.
- 转录后的基因调节,主要是通过RNA结合蛋白,对T. brucei.至关重要.
- DRBD18是一种T. bruceiRNA结合蛋白,涉及转化控制.
研究的目的:
- 研究DRBD18在T. brucei.的翻译控制中的作用.
- 确定DRBD18如何影响mRNA翻译效率和核糖体占用率.
- 阐明DRBD18对寄生虫分化和宿主适应的贡献.
主要方法:
- 对于DRBD18枯竭的RNA干扰 (RNAi).
- 多体概况分析以评估翻译活动.
- 核糖体剖析和RNA免疫沉/qRT-PCR用于识别目标mRNA.
主要成果:
- 由于DRBD18的枯竭,多体细胞的形状发生了变化,重型多体细胞减少了.
- 101个转录显示了翻译效率 (TE) 的变化,其中41个下降,60个增加.
- DRBD18抑制了前循环形式特定的转录,并增强了血液/元循环形式的转录.
结论:
- DRBD18在T. brucei.中的特定mRNA的翻译控制中发挥着重要作用.
- 通过差异性mRNA翻译,DRBD18有助于维持前循环寄生虫状态.
- DRBD18的功能对于T. brucei在整个生命周期的适应和生存至关重要.
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