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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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相关实验视频

Updated: Jul 16, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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一个受控制的对象芯片用于反射共焦显微镜,具有亚衍射极限分辨率.

Jun He1, Dong Zhao1, Hong Liu2

  • 1Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, China.

Nature communications
|September 20, 2023
PubMed
概括

平面衍射镜头通过优化无序结构来实现超高分辨率的聚焦. 一种新方法平衡成像和聚焦,使超紧显微镜能够用于超高分辨率成像.

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科学领域:

  • 光学是什么?光学是什么?光学是什么?
  • 纳米技术纳米技术
  • 显微镜的使用方法

背景情况:

  • 平面衍射镜头 (PDL) 提供微衍射极限聚焦,但由于结构混乱,会损害广场成像.
  • 现有的PDL在显微镜应用中面临局限性,原因是被摧毁了广场成像能力.

研究的目的:

  • 引入信息 (S) 作为量化PDL障碍的指标.
  • 预测一个平衡点平衡广场成像 (斯特雷尔比率) 和次衍射极限聚焦.
  • 设计和制造一个PDL目标芯片,以提高性能.

主要方法:

  • 使用信息 (S) 来评估与标准弗雷内尔区域板块的结构偏差.
  • 应用变化理论来确定最佳镜头设计的平衡点.
  • 设计一个具有1毫米焦距的PDL目标芯片,为预测的平衡点进行了优化.

主要成果:

  • 一个制造出来的PDL芯片实现了0.44λ的低衍射极限焦点大小.
  • 实验性成像显示分辨率高达4000行对/毫米.
  • 设计的芯片是报告中最接近理论平衡点的PDL.

结论:

  • 开发的信息方法有效地平衡了PDL的成像和聚焦能力.
  • 制造的PDL芯片使得超紧的反射共焦显微镜能够用于超高分辨率成像.
  • 这项工作克服了PDL中无序结构的局限性,扩大了它们在先进成像中的潜力.