鱼类聚氨酸转移酶产生各种酸聚合物
Mathieu Decloquement1, Marzia Tindara Venuto2, Virginie Cogez1
1Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, 59000, Lille, France.
Scientific reports
|September 20, 2023
概括
鱼类聚氨基转移酶,特别是Coregonus maraena ST8Sia IV,与人类对应物相比,具有明显的基质特异性. 这些鱼类酶可以产生多种多样化的聚酸异聚合物,这表明生物功能已经进化.
科学领域:
- 葡萄糖生物学 葡萄糖生物学
- 酶学 是一种酶学.
- 进化生物学 进化生物学
背景情况:
- 人类聚氨基转移酶ST8Sia II和ST8Sia IV合成聚氨酸 (polySia) 均聚合物,对生理过程至关重要.
- 鱼在卵巢和卵表面表现出异质的酸聚合物,其中涉及三个潜在的基因 (st8sia4,st8sia2-r1,st8sia2-r2).
- 多化在各个物种的各种生理和发育过程中起着至关重要的作用.
研究的目的:
- 克隆,表达和生物化学特征鱼类多化转移酶,专注于Coregonus maraena ST8Sia IV.
- 与人类酶相比,研究鱼类聚氨酸转移酶的基质特异性和产品多样性.
- 探索鱼类中聚酸生物功能的潜在演变.
主要方法:
- 在HEK293细胞中克隆和重组三种鱼类聚氨酸转移酶的表达.
- 使用MicroPlate Sialyltransferase Assay和非天然的CMP-SiaNAl基质,对C. maraena ST8Sia IV进行生物化学表征.
- 在无细胞和基于细胞的系统中使用自然基质 (CMP-Neu5Ac,CMP-Neu5Gc,CMP-Kdn) 进行多化分析.
- 使用抗polySia抗体735,内核氨基酶N和HPLC进行聚化产品的结构分析.
主要成果:
- 证明了C. maraena ST8Sia IV的酶活性,并优化了多化条件.
- 突出了人类和鱼类聚氨基转移酶之间的明显基质特异性.
- 揭示了鱼类酶产生异质聚酸异聚合物的能力,与人类同聚合物合成不同.
- 结构分析证实了由鱼类酶产生的多链的独特性质.
结论:
- 沙门类多基转移酶与它们的人类正体相比,具有独特的生化特性和基质偏好.
- 鱼类酶产生异聚合物的能力表明,在聚酸功能和生物合成方面存在进化差异.
- 科雷戈努斯马拉纳 (Coregonus maraena ST8Sia IV) 是一种关键酶,用于修改具有多种多Sia链的糖蛋白,为鱼类特定的生物作用提供了洞察力.
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