在多发性骨髓瘤中,对内质网膜与压力相关的特征进行整合性分析
Chengyu Wu1, Mei Liu2, Jia Liu1
1Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
The journal of gene medicine
|September 21, 2023
概括
我们确定了九个与内质网膜压力相关的基因 (ERS-RG) 作为多发性骨髓瘤 (MM) 的潜在诊断生物标志物. 这些ERS-RG签名可能有助于临床开发新的MM诊断和治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 多发性骨髓瘤 (MM) 是一种无法治愈的血细胞恶性瘤.
- 毫米细胞表现出高的内等质网膜应激 (ERS),并依赖其生存反应.
- 识别与ERS相关的分子特征对于MM的诊断和治疗至关重要.
研究的目的:
- 为了识别多发性骨髓瘤 (MM) 的ERS相关基因特征.
- 评估MM中已识别的ERS相关基因的诊断价值.
- 探索MM中与ERS相关的潜在治疗点和分子相互作用.
主要方法:
- 从基因表达总量数据库下载和分析了三个MM数据集.
- 确定了与ERS相关的差异表达基因 (ERDEGs),并进行了基因本体学丰富分析.
- 构建的蛋白质-蛋白质相互作用,转录因子-mRNA,miRNA-mRNA和药物-mRNA网络.
- 使用接收器运行特征分析评估免疫细胞透和诊断值.
- 使用定量聚合酶链反应 (qPCR) 验证的ERDEG表达.
主要成果:
- 在MM中识别了九个ERDEG的签名.
- ERDEGs涉及到内质网膜膜功能.
- 建立了互动网络,揭示了ERDEGs的潜在分子合作伙伴和药物点.
- 在ERDEGs和免疫细胞透之间展示了显著的相关性.
- 证实了这些潜在的MM生物标志物的良好诊断值.
- 与健康捐赠者相比,在MM细胞中观察到ERDEGs的差异表达.
结论:
- 已发现的9个与ERS相关的基因 (CR2,DHCR7,DNAJC3,KDELR2,LPL,OSBPL3,PINK1,VCAM1,XBP1) 是MM的潜在生物标志物.
- 这种基因特征有望促进多发性骨髓瘤的临床诊断和治疗.
- 对这些ERDEG的进一步研究可能会揭示MM的新疗法策略.
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