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染色体重塑分析显示,RdDM通路对玉米中低应激反应

Bowen Luo^1,2,3, Ziqi Zhang^2,3, Binyang Li^2,3

¹State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, 611130, Sichuan, China.

The Plant journal : for cell and molecular biology

|September 21, 2023

概括

低压力会改变植物染色质结构,影响基因调节和根部发育. 这项研究揭示了通过3D基因组学和RNA指导的DNA甲基化途径对植物信号传递的新见解.

科学领域:

植物生物学植物生物学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

植物 (Pi) 信号传递对生长和发育至关重要.
现有的Pi信号研究主要集中在基因相互作用上,忽视了3D染色体结构的作用.
了解3D基因组在Pi信号传递中的作用对于扩展当前知识至关重要.

研究的目的:

为了研究低 (Pi) 应激对植物染色体3D结构的影响.
探索RNA指导的DNA甲基化 (RdDM) 途径和在Pi压力下的染色质组织之间的关系.
阐明植物适应低Pi条件的新机制.

主要方法:

在低Pi处理下分析染色质体积,绝缘分数和TAD类域.
甲基化概况 (RdDM水平) 和RdDM位点分布在TAD类边界附近的分析.
对RdDM通路基因和ChIP-seq的基因表达分析,以确定ZmDDM1A结合标.

主要成果:

低Pi治疗增加了染色质体积,绝缘分数和TAD类域.
RdDM水平在TAD类边界达到峰值,在保存边界观察到特定的转移.
RdDM路径突变的ddm1a表现出增强的低Pi耐受性,ZmDDM1A结合与Pi和根发育基因有关.

结论:

植物3D染色体结构由低Pi应激动态调节,通过RdDM通路影响基因表达.
该研究为使用3D基因组学分析生物过程提供了一个框架,扩大了对植物Pi信号的理解.
这些发现突出了染色体组织,RdDM和营养压力下的根特征之间的相互作用.

关键词:

这就是Hi-C.通过RNA指导的DNA甲基化.类似 TAD 的域名.染色体空间结构的空间结构低压力低压力玉米玉米玉米是一种

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