在 Sarcocystis sp. 的研究中, 在巴西,由常见的博阿蛇 (Boa constrictor) 产生的
Taynar Lima Bezerra1, Rogério Fernando de Jesus1, Rodrigo Martins Soares2
1Department of Anatomy, Pathology and Clinics, Universidade Federal da Bahia, Escola de Medicina Veterinária e Zootecnia, Avenida Adhemar de Barros, 500, Ondina, Salvador, 40170-110, Bahia, Brazil.
International journal for parasitology. Parasites and wildlife
|September 21, 2023
概括
在巴西的普通波亚 (Boa constrictor) 中发现了一种新的Sarcocystis物种. 这一发现扩大了对南美Sarcocystis多样性和潜在宿主-寄生虫关系的了解.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 分子生物学分子生物学
- 类学 类学 类学 类学
背景情况:
- Sarcocystis属包括大约200个物种,其中25种已知可以感染蛇.
- 在蛇中发现的Sarcocystis nesbitti和Sarcocystis singaporensis对人类和动物的健康有重大影响.
- 巴西拥有高度的蛇生物多样性,但巴西蛇中的Sarcocystis物种在很大程度上仍然没有特征.
研究的目的:
- 调查巴西常见的波亚 (波亚) 中的Sarcocystis物种的存在和遗传特征.
- 为了确定在 Boa constrictor 中发现的 Sarcocystis 是否代表了一种新的物种.
- 探索这个新发现的Sarcocystis物种的潜在中间宿主.
主要方法:
- 检查65只常见的波亚 (Boa constrictor) 的便,检查它们的 Sarcocystis sporocysts.
- 脊髓囊泡尺寸的形态测量分析.
- 使用PCR和四种遗传标记物 (cox1,18S,ITS1和28S) 的测序进行分子表征.
- 在小鼠和Rattus norvegicus中进行生物测试,以确定中间宿主.
主要成果:
- 在1.53% (1/65) 的考察Boa constrictor中检测到sarcocystis杂囊.
- 形态测量和分子分析表明,这些囊属于一种新的Sarcocystis物种.
- 遗传测序显示,与阿根廷动物Lagostomus maximus的Sarcocystis具有很高的相似性,这表明存在潜在的宿主-寄生虫联系.
- 在生物测试中,在小鼠或Rattus norvegicus中没有检测到任何寄生虫阶段.
结论:
- 常见的 (Boa constrictor) 可能是新型Sarcocystis物种的最终宿主.
- 拉戈斯托莫斯最大或相关的动物是这种新的Sarcocystis物种的可能中间宿主.
- 这一发现凸显了对巴西蛇中的Sarcocystis多样性及其生态作用的进一步研究的需要.
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