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马尔托尔通过调节B16F10细胞中的PD-L1信号通路具有抗癌作用

Na-Ra Han^1,2, Hi-Joon Park³, Seong-Gyu Ko^2,4

¹College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.

Frontiers in pharmacology

|September 21, 2023

概括

马尔托尔是一种具有抗氧化特性的化合物,通过降低免疫检查点PD-L1.1的调节,有效地抑制黑色素瘤的生长. 这种作用增强了T细胞的反应,克服了免疫疗法耐药性,这表明马尔托尔是潜在的黑色素瘤治疗方法.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
皮肤病学皮肤病学

背景情况:

黑色素瘤是一种高死亡率的皮肤癌,通过免疫疗法改善了治疗方法,特别是免疫检查点调节.
马尔托尔具有抗氧化和抗炎性质,但其抗黑色素瘤潜力和通过免疫检查点的机制在很大程度上尚未被探索.

研究的目的:

为了研究马尔托尔的抗黑色素瘤潜力.
通过调节免疫检查点,特别是PD-L1.1,探索马尔托尔的作用机制.

主要方法:

量化PCR (qPCR),免疫斑块和免疫光被用于分析编程死亡 1 (PD-L1) 表达式.
用CTLL-2细胞评估了黑色素瘤细胞对T细胞的敏感性,使用细胞毒性,细胞活力和互白素-2 (IL-2) 试验.
用马尔托尔和/或西斯普拉丁治疗B16F10黑色素瘤细胞,以评估协同效应和亡诱导.

主要成果:

马尔托尔降低了黑色素含量,铁酶活性,以及铁酶和铁酶相关蛋白1在黑色素瘤细胞中的表达.
马尔托尔通过激活caspase-3和PARP.抑制了B16F10的增殖,诱导细胞循环停止,并增加了细胞亡.
马尔托尔通过抑制STAT1酸化来抑制IFN-γ和西斯普拉丁诱导的PD-L1表达,增强西斯普拉丁的细胞毒性并通过增加IL-2的产生增加T细胞介导的黑色素瘤破坏.

结论:

马尔托尔通过降低PD-L1表达的调节,有效地限制黑色素瘤的生长.
马尔托尔增强了T细胞介导的抗癌反应,并克服了PD-L1介导的免疫疗法抵抗.
马尔托尔显示出作为治疗黑色素瘤治疗的有效治疗剂的潜力.

关键词:

B16F10 细胞 B16F10 细胞在CTLL-2细胞中.在PD-L1中.西斯普拉丁 (cisplatin) 是一种制剂.马尔托尔马尔托尔是什么意思黑色素瘤是一种黑色素瘤.

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