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染色体水平的基因组组合,用于血管精子Silene conica

Peter D Fields^1,2, Melody M Weber¹, Gus Waneka¹

¹Department of Biology, Colorado State University, Fort Collins, Colorado, USA.

bioRxiv : the preprint server for biology

|September 21, 2023

概括

我们展示了Silene conica的高质量基因组组,揭示了它的染色体结构和大型线粒体基因组. 这个资源有助于在Silene属的进化和基因组研究.

科学领域:

植物基因组学植物基因组学
进化生物学是进化的生物学.
生态生态学生态生态学

背景情况:

有限的高质量基因组序列存在于血管苗属Silene,阻碍了生态和进化研究.
沉默的物种是研究生态,进化和遗传学的重要模型生物.
之前的研究表明,与该属的祖先状态相比,静音物种的基染色体数量减少了.

研究的目的:

为Silene conica.提供染色体级的基因组组件.
在Silene中促进基因组研究,包括比较分析.

主要方法:

使用了PacBio的HiFi,Hi-C和Bionano光学映射技术.
产生了862 Mb的染色体级组件,其间隙含量低 (~1%).

主要成果:

该组件由10个支架组成,对应于Silene conica.的染色体数.
沉默的体具有异常大的线粒体基因组 (>11 Mb),其序列起源基本上是未知的.
核DNA转移不太可能是线粒体基因组扩张的主要原因.

结论:

Silene conica的染色体级基因组组合为未来的Silene基因组研究提供了宝贵的资源.
这种组合将促进与相关物种进行比较分析,特别是那些最近进化了性染色体的物种.
了解Silene中核和细胞质基因组的共同进化,通过这种基因组资源得到了增强.

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