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Updated: Jul 16, 2025

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
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In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

Published on: July 23, 2010

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DropBlot:单细胞西部抹除化学固定的癌细胞

Yang Liu, Amy E Herr

    bioRxiv : the preprint server for biology
    |September 21, 2023
    PubMed
    概括

    这项研究介绍了DropBlot,这是一个微流体平台,用于在单个固定细胞中检测敏感蛋白质. 它可以从存档的组织中进行高分辨率的蛋白质组分析,从而推进翻译研究.

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    Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

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    Western Blotting01:15

    Western Blotting

    Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
    The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
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