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基于DNA桥接双黄金纳米粒子的免疫染色学,用于双模式检测甲素A

Youxin Qiu¹, Zhaoying Liu¹, Yuhao Mao¹

¹School of the Environment and Safety Engineering, Institute of Environmental Health and Ecological Security, Jiangsu University, Zhenjiang, P. R. China.

Journal of food science

|September 21, 2023

概括

一个新的DNA桥接金纳米粒子免疫染色学条带 (ICS) 提供了增强的双模式检测甲毒素A (OTA). 这种方法提高了OTA污染的半量化和量化敏感度.

科学领域:

分析化学分析化学
纳米技术纳米技术
生物感应是一种生物感应.

背景情况:

毒素A (OTA) 是一种普遍存在的真菌毒素,具有重大健康风险.
准确而敏感的OTA检测方法对于食品安全至关重要.
传统的检测方法可能耗时,需要专门的设备.

研究的目的:

开发一种新的免疫染色谱条 (ICS) 用于OTA的双模式检测.
为了提高OTA检测的灵敏度和效率,使用DNA桥接金纳米粒子.
为半量化和量化OTA提供可靠和实用的方法.

主要方法:

使用DNA桥接双金纳米粒子 (AuNPs) 制造一个增强的ICS.
在40nm AuNPs上阻塞斯特雷普塔维丁,在20nm AuNPs上阻塞OTA抗体/生物化DNA-SH.
评估ICS用于OTA的双模式检测 (半量化和量化).

主要成果:

通过肉眼实现了0.06 ng/mL的半量化检测极限 (半Q-LOD) (8倍增强).
通过现场阅读器 (增强3倍) 达到0.03 ng/mL的量化检测极限 (Q-LOD).
证明了高的特异性,可靠性和可行性,结果与HPLC-MS/MS相对应.

结论:

开发的DNA桥接双AuNP ICS为OTA提供了灵敏高效的双模式检测平台.
这种新的ICS模式比OTA污染监测的传统方法提供了宝贵的改进.
该技术作为推进ICS敏感性和适用于食品安全的参考.

关键词:

生物化DNA是生物化DNA.双重黄金纳米粒子的使用.双模式检测检测双模式检测增强的免疫染色学增强的免疫染色学 ochratoxin A 是一种毒素.

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