对于门诊患者的Helicobacter pylori抗生素耐药性的表型和基因型分析:一个回顾性研究
Mengqiu Xiong1, Hend Sadeq Mohammed Aljaberi1, Nida Khalid Ansari1
1Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University , Nanjing, China.
Microbiology spectrum
|September 21, 2023
概括
在南京的门诊患者中,Helicobacter pylori的抗生素耐药性对米特罗尼达,克拉里胺和勒沃素很高. 基因型定型可以预测对克拉里素和勒沃素的耐药性,帮助治疗决策.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 临床医学 临床医学
背景情况:
- 杆菌感染很普遍,导致严重的胃疾病.
- 了解抗生素耐药性模式对于有效的H. pylori根除疗法至关重要.
- 之前的研究强调了不同的抗药率,需要局部化数据.
研究的目的:
- 在门诊患者中调查H. pylori的抗生素耐药性表型和基因型.
- 探索基因型和表型H. pylori抗生素耐药性的一致性.
- 为基于耐药性概况的临床治疗策略提供指导.
主要方法:
- 对从南京门诊患者中分离出来的3306种H. pylori菌株进行了回顾性分析.
- 抗生素耐药性表型通过Kirby-Bauer盘扩散确定.
- 使用实时聚合酶链反应分析的抗生素耐药性基因型.
主要成果:
- 对甲尼达 (74.58%),克拉里胺素 (48.61%) 和勒沃素 (34.83%) 观察到高耐药率.
- 特定的基因突变 (rdxA,23S rRNA,gyrA) 与对分别对美特罗尼达,克拉里思罗米辛和勒沃素的耐药性相关.
- 在表型和基因型对克拉里菌素和勒沃素的耐药性之间发现了良好的一致性.
结论:
- 杆菌对美特罗尼达,克拉里素和勒沃素的耐药性是南京门诊患者的一个重要问题.
- 基因型分析可以可靠地预测表型耐克性对克拉里菌素和levofloxacin.
- 这些发现支持对H. pylori感染的基因型导向治疗选择.
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