三个MALDI-TOF质谱系统与16S rRNA基因测序的对比比较
Karl Dichtl1, Isabel Klugherz1, Hanna Greimel1
1Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz , Graz, Austria.
Journal of clinical microbiology
|September 21, 2023
概括
在微生物学诊断中,矩阵辅助激光脱/离子化飞行时间质谱 (MALDI-TOF MS) 系统至关重要. 这项研究发现,MALDI Biotyper,EXS2600和Vitek MS提供了可比性能,与16S rRNA测序高度一致.
科学领域:
- 临床微生物学 临床微生物学
- 诊断技术 诊断技术 诊断技术
- 细菌的鉴定 细菌的鉴定
背景情况:
- 矩阵辅助激光脱吸/电离飞行时间质谱 (MALDI-TOF MS) 已经改变了微生物诊断.
- 已建立的系统包括MALDI Biotyper和Vitek MS,EXS2600是新进入者.
研究的目的:
- 评估和比较三个MALDI-TOFMS系统的诊断性能:MALDI生物型,EXS2600和维特克MS.
- 为了评估它们与难以识别的细菌的16S rRNA基因测序的准确性.
主要方法:
- 在三个MALDI-TOFMS平台上,共测试了356种细菌分离物.
- 使用了直接涂抹和简单的酸提取方法.
- 结果与16S rRNA基因测序进行了基准测试.
主要成果:
- 对98.6% (MALDI生物型),94.4% (EXS2600) 和93.3% (Vitek MS) 的分离物获得了有效的鉴定结果.
- 对测序的一致性很高:98.9% (MALDI生物型),98.5% (EXS2600) 和99.7% (Vitek MS) 的结果.
- 在单个物种层面的错误识别率为0% (MALDI生物型),2.6% (EXS2600) 和1.1% (Vitek MS).
结论:
- 所有三个MALDI-TOF MS系统都在细菌识别方面表现出可比且可靠的性能.
- 在性能指标中观察到微小的差异,但所有系统都适合临床诊断实验室.
- 马尔迪-托夫MS仍然是常规微生物识别的强大工具,有助于及时诊断患者.
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