在朝向迁移的Xenopus gastrula mesendoderm中极化接触行为
Martina Nagel1, Rudolf Winklbauer1
1Department of Cell and Systems Biology, University of Toronto, Toronto, Canada.
The International journal of developmental biology
|September 21, 2023
概括
细胞粘附和脱离控制集体细胞迁移. 这项研究确定了调节Xenopus mesoderm爆炸性融合和排斥的三个模块,揭示了细胞行为如何促进定向迁移.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 集体细胞迁移对于发育和组织修复至关重要.
- 了解细胞-细胞粘附和脱离机制是控制细胞运动的关键.
研究的目的:
- 为了研究在集体细胞迁移过程中活性细胞脱离的调节.
- 在Xenopus gastrula中鉴定控制细胞-细胞粘附和排斥的分子模块.
主要方法:
- 使用了Xenopus gastrula中皮层在ectoderm条件下的基板上的爆炸物.
- 分析了探索者接触行为,包括排斥和融合.
- 研究了PDGF-A,Xwnt6,Xfz7,Pak1,ephrinB1和Dishevelled (Dvl) 信号通路的作用.
主要成果:
- 爆炸物在前后对齐时,在侧面碰撞和融合时表现出排斥力.
- 发现PDGF-A信号以极化方式抑制接触诱导的乳类塌.
- 与Dvl一起作用的Wnt受体Xfz7和ephrinB1,控制了爆炸性融合和组织分离.
- 基底结合的指导线索逆转了爆炸性排斥/融合反应,影响了拉梅利波迪亚的定向接触抑制.
结论:
- 三个不同的分子模块在集体迁移期间调节细胞-细胞脱离和粘附.
- 基质结合的线索和已识别的模块协同作用,促进优先的前后粘附和定向的中皮层迁移.
- 研究结果提供了有关组织形态发生和集体细胞行为机制的见解.
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