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对Cochliomyia hominivorax U6基因促进体的鉴定和功能分析
Rossina Novas1,2, Tatiana Basika1,2, Megan E Williamson1
1Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, North Carolina, USA.
Insect molecular biology
|September 21, 2023
概括
研究人员评估了新世界螺丝虫 (Cochliomyia hominivorax) U6基因促进器对CRISPR/Cas9基因驱动系统的活性. 乔姆U6_b促销者表现出最高的活性,为害虫控制提供了潜力.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 新世界螺丝虫 (Cochliomyia hominivorax) 是一个重要的牲畜害虫,在北美和中美洲使用无菌昆虫技术成功根除了它.
- 对于南美洲的Cochliomyia hominivorax种群,需要更具成本效益的遗传控制方法.
- 克里斯普尔/卡斯9基因驱动系统为有效的人口抑制提供了一个有希望的途径.
研究的目的:
- 开发和验证一种体内测试,以评估Cochliomyia hominivorax U6基因促进者的活性.
- 确定最活跃的U6基因促进子,以在基于CRISPR/Cas9的基因驱动系统中潜在使用,该系统针对Cochliomyia hominivorax.
主要方法:
- 在使用相关的吹Lucilia cuprina的胚胎建立了体内测试.
- 含有U6促进器导向RNA结构和Cas9源 (蛋白质或DNA) 的等离子体DNA被注入胚胎中.
- 通过评估化幼虫的目标基因中的特定位点突变来量化指导RNA促进因子活性.
主要成果:
- 评估了七种Cochliomyia hominivorax U6基因促进体的活性.
- 在测试的促销者中,Chom U6_b促销者表现出最高的活性.
- 这表明它适合在基因驱动系统中驱动指导RNA表达.
结论:
- 开发的体内测试有效地测量了Cochliomyia hominivorax U6基因促进者的活性.
- 乔姆U6_b促销器是开发基于CRISPR/Cas9的基因系统的强有力的候选人,用于控制Cochliomyia hominivorax.
- 这些发现为新的遗传策略铺平了道路,以管理这个经济上重要的牲畜害虫.
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