非淋巴体体皮RNA通路确保丝虫的性分化,幼虫生长和翅膀发育
Takashi Kiuchi1, Keisuke Shoji2, Natsuko Izumi2
1Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, Japan.
PLoS genetics
|September 21, 2023
概括
与PIWI相互作用的RNAs (piRNAs) 对生殖系中的基因组完整性至关重要. 在丝虫中,体性piRNAs对于正常的幼虫生长,发育和性差异化至关重要,突出了它们在繁殖之外的更广泛作用.
科学领域:
- 表观遗传学和基因调控
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 与PIWI相互作用的RNAs (piRNAs) 和PIWI蛋白质通过抑制元动物生殖细胞中的转体子来保护基因组完整性.
- 虽然Drosophila Piwi在体组织中发挥了作用,但由于表型有限,其他PIWI蛋白 (Aub,Ago3) 在非淋巴体组织中的重要性仍然不清楚.
研究的目的:
- 研究丝虫 (Bombyx mori) 的PIWI蛋白,Siwi和BmAgo3在非阴茎体组织中的功能.
- 阐明体质piRNA生物发生在Bombyx mori发育中的作用.
主要方法:
- 在Bombyx mori.中产生Siwi和BmAgo3功能丧失突变体.
- 突变丝虫的表型分析,包括生长,发育和性差异化.
- 转录组分析以评估突变者的基因表达变化.
主要成果:
- 功能丧失的Siwi和BmAgo3突变体表现出幼虫生长延迟,未能发展成成年.
- 突变者在翅膀发育和性差异化方面表现出显著的缺陷.
- 转录组分析揭示了体质piRNA生物生成突变体中转子和宿主基因的异常表达,表明严重的生长缺陷.
结论:
- 在Bombyx mori中,体性piRNA对幼虫正常发育至关重要,包括生长,翅膀形成和性差异化.
- 身体组织中的piRNA途径在调节转子子体活性和宿主基因表达方面发挥着关键作用.
- 这些发现强调了Bombyx mori.中的非淋巴体体性piRNAs在发育中的重要作用.
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