相关实验视频

Updated: Jul 16, 2025

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

亮点:一种光遗传系统,用于控制植物中高分辨率的基因表达

Bo Larsen¹, Roberto Hofmann¹, Ines S Camacho²

¹Sainsbury Laboratory, University of Cambridge, Cambridge, United Kingdom.

|September 21, 2023

概括

亮点器是植物的新型光遗传系统,可以通过光来精确控制基因表达. 这项技术提供了高分辨率的基因诱导,以推进植物生物学和改善作物.

科学领域:

植物生物学植物生物学
合成生物学合成生物学
视觉遗传学视觉遗传学

背景情况:

光遗传学提供了精确的生物控制,但由于光环境和与内源系统的交叉声,在植物中面临挑战.
现有的光遗传工具通常需要外部辅助因子或与本地植物信号通路相互作用.

研究的目的:

开发一种新的,基因编码的光遗传系统,用于植物中的应用.
克服当前植物光遗传工具的局限性,实现高分辨率的基因表达控制.

主要方法:

开发了Highlighter,一种基于蓝藻细菌CcaS-CcaR系统的合成光门基因表达系统.
为植物使用重新设计和重新设计了CcaS系统,利用内源性植物染色素和一种新的蓝光反应.
在Nicotiana benthamiana中部署的亮点用于光蛋白表达和下游基因标的光遗传控制.

主要成果:

证明了重新设计的CcaS系统的绿色/红色和蓝色光依赖光交换的成功.
使用光在邻近的植物细胞中实现了前所未有的基因表达的时空控制.
通过改变光谱组成,展示了植物免疫和色素生产的光遗传学调节.

结论:

亮点器为植物提供了强大的,基因编码的光遗传解决方案.
这个系统使高分辨率的基因诱导成为可能,进步了植物科学的基础.
突出显示器为改善作物和生物技术应用提供了新的途径.

更多相关视频

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

Published on: August 19, 2021

相关概念视频

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.