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病原性细菌利用转移素受体转细胞化穿越血脑屏障

Zhihui Cheng^1,2, Yangyang Zheng^1,3, Wen Yang^1,3

¹The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071, China.

Proceedings of the National Academy of Sciences of the United States of America

|September 21, 2023

概括

常见的细菌使用一种独特的囊泡融合过程,通过转移素受体 (TfR) 转细胞突变穿越血脑屏障 (BBB). 这种机制涉及先天的免疫信号和特定的蛋白质复合体的形成,以便细菌进入.

科学领域:

神经科学是一个神经科学.
免疫学免疫学免疫学
微生物学微生物学

背景情况:

血脑屏障 (BBB) 保护大脑免受病原体的侵害,但其被脑膜炎引起的细菌所破坏的程度尚不清楚.
脑膜炎仍然是一个严重的疾病,需要研究细菌入侵机制.

研究的目的:

阐明主要细菌性脑膜炎病原体穿越人类BBB.的机制.
详细介绍细菌使用的囊泡融合和转移素受体 (TfR) 转细胞解路径.

主要方法:

使用脑微血管内皮细胞 (HBMECs) 进行了体外人类BBB模型.
使用小鼠模型在体内研究细菌BBB透.
研究了分子相互作用,包括蛋白质无处不在,复合体形成和囊泡融合.

主要成果:

确定了一种常见的囊泡融合机制,这种机制被*Streptococcus pneumoniae*,B组*Streptococcus*和*Escherichia coli*利用.
证明了细菌劫持TfR转细胞因子以进行BBB交叉.
揭示了在含有细菌的囊泡 (BCVs) 上的托尔类受体信号触发了TRAF3的修饰和特定蛋白质复合体 (RCC2,RALA,外囊子复合体I) 的形成.
显示的SEC6和SNAP23调解了BCVs与TfR囊泡的结合和融合.

结论:

天生的免疫力激活特定的TRAF3修饰和BCVs上的HBMEC特异性蛋白质复合体.
这一过程确保了与TfR囊泡的精确识别和融合,促进了细菌透BBB的过程.

关键词:

这是细菌性脑膜炎.主体病原体相互作用通过BBB的透.转移林受体的转细胞化囊泡融合是什么意思囊泡融合

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