甲氨酸生物合成酶MoMet2是通过通过减少5mC修饰来促进毒性基因表达来促进大米爆发真菌病原性所需的
Huimin Li1, Pengcheng Mo1, Jun Zhang1
1Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
PLoS genetics
|September 21, 2023
概括
抗真菌杀菌剂的耐药性威胁到农作物. 科学家们通过破坏其发育和毒性,确定了同类氨酸转移酶 (HOA) 作为Magnaporthe oryzae的新目标,这种真菌会导致大米爆发.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 菌类遗传学 菌类遗传学
背景情况:
- 抗真菌杀菌剂耐药性对全球作物生产构成重大威胁.
- 识别新的抗真菌点对于制定有效的疾病管理策略至关重要.
- 麦格纳波菌 (Magnaporthe oryzae) 导致大米爆发,这是一种破坏性疾病,影响全球大米产量.
研究的目的:
- 调查同类酸转移酶 (HOA) 作为Magnaporthe oryzae.一个潜在的抗真菌标的功能.
- 了解HOA在真菌发育,毒性和表观遗传调节中的作用.
- 评估向HOA对控制大米爆炸的有效性.
主要方法:
- 基因删除突变体 (∆Momet2, ∆Cys2) 是为了研究HOA功能而创建的.
- 分析了 metionin 和 S-adenosylmethionine 的生物合成途径.
- 量化了5-甲基细胞素 (5mC) 修饰水平和基因表达.
- 用宿主诱导的基因沉默 (HIGS) 来准HOA基因.
主要成果:
- 删除MoMET2和MoCYS2基因减弱了M. oryzae的发展和毒性.
- HOA 干扰改变了氨酸和S-adenosylmethionine 的生物合成.
- ∆Momet2突变体表现出增加的5-甲基细胞因基因修饰,抑制了致病性基因.
- 针对MoMET2和MoCYS2的HIGS有效控制了水爆炸.
结论:
- 荷莫塞林O-乙转移酶 (HOA) 在M. oryzae的发育和毒性中发挥着关键作用.
- HOA是开发新型反大米爆炸策略的有希望的目标.
- 针对HOA提供了一种潜在的解决方案,以对抗大米种植中的杀真菌剂耐药性.
相关概念视频
01:56Riboswitches
8.2K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.2K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
01:28Biosynthesis of Nucleic Acids
67
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
67
01:23Transcriptional Regulation: Riboswitches
58
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
58
01:40Overview of Metabolism
30.7K
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
30.7K
02:23Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K