相关实验视频

Updated: Jul 16, 2025

Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration

Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration

Published on: November 2, 2020

ErbB4处理参与OGD/R诱导的神经元损伤

Aiqing Deng¹, Shouyan Wang², Jianxin Qin²

¹Department of Pharmacy, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, People's Republic of China.

Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association

|September 21, 2023

概括

ErbB4通过减少细胞死亡和反应性氧物种,保护大脑免受氧-葡萄糖剥夺/再输液 (OGD/R) 损伤. 这种神经保护作用涉及到Adrb1,Adrb2,Ldlr和Dab2基因表达的变化.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
遗传学遗传学是一种遗传学.

背景情况:

在氧气-葡萄糖剥夺/再注射 (OGD/R) 脑损伤后,ErbB4基因表达被改变.
在脑缺血中ErbB4的确切功能和潜在机制在很大程度上仍然未被描述.

研究的目的:

研究ErbB4在OGD/R诱导的大脑损伤中的神经保护作用.
阐明 ErbB4 发挥保护作用的分子机制.

主要方法:

建立体外和体内脑缺血/再输血 (I/R) 损伤模型.
使用MTT,TUNEL和DCFH-DA试验评估细胞活力,细胞亡和反应性氧物种 (ROS) 生产.
生物信息学分析,RNA-Seq,定量RT-PCR和西部涂抹,以识别关键基因并分析表达水平.

主要成果:

在大脑I/R损伤模型中,ErbB4表达显著降低.
ErbB4表达与Adrb1,Adrb2,Ldlr和Dab2相关,在OGD/R后观察到差异性mRNA表达.
通过神经调节素1 (NRG1) 介导的ErbB4激活增强了细胞存活率,减少了细胞亡,减少了ROS的产生,而ErbB4 siRNA则逆转了这些效应.

结论:

ErbB4对OGD/R损伤具有显著的神经保护作用.
ErbB4的保护作用与其与膜相关的片段和Adrb1,Adrb2,Ldlr和Dab2表达的调制有关.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.大脑缺血/再输血这就是ErbB4的意思.氧气-葡萄糖剥夺/再输血一次性中风,中风.

更多相关视频

A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

Published on: May 5, 2018

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Published on: August 17, 2012

相关概念视频

Neurogenesis and Regeneration of Nervous Tissue

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...