Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.2K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
Sanger Sequencing01:57

Sanger Sequencing

754.7K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.7K
Next-generation Sequencing03:00

Next-generation Sequencing

89.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
89.8K
RNA-seq03:21

RNA-seq

10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Quantum gate algorithm for reference-guided DNA sequence alignment.

Computational biology and chemistry·2023
Same author

Regulating the quorum sensing signalling circuit to control bacterial virulence: in silico analysis.

IET systems biology·2011
Same author

[An unrecognized oesophageal rupture].

Annales francaises d'anesthesie et de reanimation·2007
Same author

Potential use of therapies for osteoporosis to treat neurological diseases of elderly people.

Medical hypotheses·2005
Same author

Activity and resistance of iron-containing amorphous, zeolitic and mesostructured materials for wet peroxide oxidation of phenol.

Water research·2005
Same author

Association of Neisseria cinerea with ocular infections in paediatric patients.

The Journal of infection·1998

相关实验视频

Updated: Jul 16, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.1K

量子算法 de novo DNA 序列组装基于量子走在图形上的量子步行.

G D Varsamis1, I G Karafyllidis2, K M Gilkes3

  • 1Department of Electrical and Computer Engineering, Democritus University of Thrace, Xanthi, 67100, Greece.

Bio Systems
|September 21, 2023
PubMed
概括

我们开发了一种新的量子算法,用于 de novo DNA 序列组装,解决了在重叠图中找到路径的 NP 难题. 这种量子方法为更高效的DNA组装方法提供了潜在的途径.

关键词:
DNA组装组件的组装在DNA测序过程中,DNA测序量子算法中的量子算法量子计算是一种量子计算.

更多相关视频

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.7K
Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

8.7K

相关实验视频

Last Updated: Jul 16, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.1K
Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.7K
Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

8.7K

科学领域:

  • 计算生物学 计算生物学
  • 量子计算是一种量子计算.
  • 生物信息学是一种生物信息学.

背景情况:

  • 对于基因组学来说,De novo DNA 序列组装至关重要.
  • 这个问题是计算密集的,被归类为NP-hard.
  • 目前的方法依赖于在重叠图中找到路径.

研究的目的:

  • 开发一个量子算法,用于新的DNA组装.
  • 为了利用量子步行,在重叠图中有效地找到路径.
  • 探索一个计算上具有挑战性的生物信息学问题的量子解决方案.

主要方法:

  • 开发了一种使用量子步行的量子算法.
  • 实施了对重叠图形的层次分区策略.
  • 对于低等级图形的联合量子步行与高等级图形的量子哈密尔顿路径算法.
  • 使用了Qiskit来测试和模拟量子算法.

主要成果:

  • 成功测试了分区量子算法.
  • 证实了量子哈密尔顿路径算法对高层次层次的正确运行.
  • 开发了一个定制的量子步行模拟器,用于在低级图中搜索路径.

结论:

  • 拟议的量子方法为高效的 de novo DNA 组装提供了一个潜在的解决方案.
  • 这项工作可能成为未来生物信息学实用量子算法的基础.
  • 量子计算显示出在序列组装中解决NP-hard问题的前景.