在Polycomb沉声器中,Arabidopsis VEL蛋白的明显辅助作用
Elsa Franco-Echevarría1,2, Mathias Nielsen2, Anna Schulten2
1Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom.
Genes & development
|September 21, 2023
概括
多镇压复合体2 (PRC2) 辅助因子VRN5和VIN3在冷诱导的表观遗传沉默中发挥着不同的作用. VRN5对于H3K27me2沉积至关重要,而两者对于H3K27me3都必不可少,影响了开花时间的调节.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 植物分子生物学 植物分子生物学
- 染色体生物学 染色体生物学
背景情况:
- 多胞体抑制复合体2 (PRC2) 是一个关键的表观遗传调节器,参与植物和动物的基因沉默.
- 在 *Arabidopsis* 中,PRC2 调解了 *FLC* 位点的冷诱导沉默,这对于调整花期与春季至关重要.
- VEL辅助因子VRN5和VIN3与PRC2相关,但其具体功能尚未完全理解.
研究的目的:
- 阐明VRN5和VIN3在PRC2-介导的表观遗传沉默中的不同功能作用.
- 研究VEL蛋白和PRC2组件之间的相互作用的基础分子机制.
- 确定这些相互作用对基因素修饰和基因调节的影响.
主要方法:
- 在体外复制PRC2复合物.
- 生物化学测试用于研究蛋白质与蛋白质相互作用.
- 染色体免疫沉 (ChIP) 用于评估基因素修饰 (H3K27me2和H3K27me3).
- 在VRN5中保存残留物的分析. ortologs.
主要成果:
- VRN5与重建的PRC2直接关联,取决于其中心域的紧形状.
- 对于VRN5的紧形状的关键残留物在植物物种中得到保护.
- VIN3与VAL1相互作用,这是一种与*FLC*位点结合的转录抑制剂.
- 对于H3K27me2沉积,VRN5是必不可少的,而对于H3K27me3.3,VRN5和VIN3都需要.
- VRN5和VIN3与整个*Arabidopsis*基因组中的许多H3K27me3修饰的标有关.
结论:
- VEL蛋白,VRN5和VIN3,在PRC2介导的冷诱导沉声中发挥着不同的辅助作用.
- VRN5与PRC2的相互作用对于特定的基因素甲基化模式 (H3K27me2) 是至关重要的.
- 这些发现对理解PRC2功能和植物基因表达的表观遗传调节有广泛的影响.
相关概念视频
Master Transcription Regulators
2.2K
2.2K
Position-effect Variegation
6.4K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.4K
Regulation of Expression at Multiple Steps
942
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
942
Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
Regulation of Expression Occurs at Multiple Steps
3.1K
3.1K
Co-activators and Co-repressors
7.4K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.4K


