相关实验视频
Updated: May 17, 2026

08:30
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
Published on: May 16, 2012
24.6K
开发和评估MiCheck®前列腺检测临床显著前列腺癌的前列腺检测
Neal D Shore1, Dmitry M Polikarpov2, Christopher M Pieczonka3
1CUSP LLC Research Consortium, Annandale, VA.
Urologic oncology
|September 21, 2023
概括
该MiCheck®前列腺测试准确地识别出临床上显著的前列腺癌,减少不必要的活检. 该测试可以在标准的罗氏科巴斯分析仪上进行,从而改善临床工作流程.
科学领域:
- 在瘤学瘤学.
- 生物化学 生物化学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 临床需要确定患有高前列腺特异性抗原 (PSA) 的患者,需要为临床显著前列腺癌 (CSCaP) 进行前列腺活检.
- 之前开发的MiCheck®测试用于CSCaP.
- 进一步开发和整合MiCheck®测试与罗氏科巴斯标准临床化学分析仪.
研究的目的:
- 调整MiCheck®前列腺测试以用于标准临床化学分析仪.
- 使用MiCheck-01临床试验的样本来描述测试的性能.
主要方法:
- 利用了来自MiCheck-01美国临床试验的358名患者样本 (46个对照组,137个非CaP,62个非CSCaP,113个CSCaP).
- 使用基于Luminex的套装测量了生长因子的血清分析剂度.
- 检查了可用标准化学分析仪测量的分析物,用于算法开发.
- 用罗氏科巴斯分析仪重新测量样品,用于最终算法开发.
主要成果:
- 开发了一个物流回归模型,包括PSA,%自由PSA,数字直肠检查 (DRE) 和人类流行病蛋白4 (HE4).
- 实现了0.85的曲线下面积 (AUC),具有95%的灵敏度和50%的特异性来区分CSCaP.
- 证明了避免高达50%的不必要活检的潜力,在诊断高等级癌症时延迟最小.
结论:
- 该MiCheck®前列腺测试有效地识别高灵敏度和负预测值 (NPV) 的CSCaP.
- 该测试适用于使用罗氏科巴斯分析仪的临床实验室.
- 该MiCheck®前列腺检测可以帮助减少不必要的前列腺活检,同时最大限度地减少诊断延迟.
相关概念视频
Imaging Studies for Cardiovascular System V: CT
Cardiac computed tomography (CT) scanning is an advanced cardiac imaging technique that utilizes CT technology, with or without intravenous (IV) contrast, to produce accurate cross-sectional virtual slices of specific areas of the heart, coronary circulation, and major blood vessels such as the aorta, pulmonary veins, and arteries. The computer processes these slices to generate three-dimensional images. Multidetector CT (MDCT) is a rapid form of CT scanning that captures multiple slices...
Imaging Studies for Cardiovascular System VI: Calcium -Scoring CT
Calcium-Scoring CT ScanA calcium-scoring CT scan, also known as coronary artery calcium (CAC) scan, detects calcium deposits in the coronary arteries. This test assesses the risk of coronary artery disease (CAD), which can lead to cardiovascular events such as angina, heart failure, and sudden cardiac arrest.A calcium-scoring CT scan is generally recommended for individuals at intermediate risk of CAD without symptoms. It includes:Men aged 40-75 and women aged 50-75: Especially those with a...
Imaging Studies III: Computed Tomography
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Imaging Studies VII: Vascular Imaging
DefinitionRenal angiography, also known as renal arteriography, is an imaging technique used to obtain a comprehensive view of blood flow and the vascular structure of blood vessels in the kidneys and surrounding areas.PurposeRenal angiography detects blood vessel abnormalities in the kidneys, such as aneurysms, stenosis, thrombosis, vascular tumors, and renal artery stenosis. It evaluates kidney function and guides interventional treatments like angioplasty or stent placement.Pre-Procedure...

