定义和识别细胞子交叉组对,以表征细胞-细胞通信模式
Chenxing Zhang1, Yuxuan Hu1, Lin Gao2
1School of Computer Science and Technology, Xidian University, Xi'an, 710071, China.
Scientific reports
|September 21, 2023
概括
这项研究引入了细胞子交叉对 (CSCP) 来分析细胞通信. CSCPs揭示了隐藏的联体受体相互作用,并预测了乳腺癌患者的免疫治疗反应.
科学领域:
- * 单细胞空间转录组学
- * 细胞间通信分析
- * 计算生物学 计算生物学
背景情况:
- *目前的细胞-细胞通信分析往往在更广泛的细胞类型层面汇总了相互作用.
- * 在组织中,单个细胞类型包括具有独特功能和信号通路的独特子组.
- *需要精细的分析来理解复杂的细胞间交叉通话.
研究的目的:
- * 开发一种识别具有强烈细胞间交叉通话的细胞子组的方法.
- * 为了研究已识别的交叉语音对中的细胞子组的空间近距离.
- *揭示细胞类型水平上掩盖的通信通路,并评估疾病的预测潜力.
主要方法:
- * 通过结合的非负矩阵因子分解来定义和识别细胞子交叉对 (CSCP).
- *分析了来自小鼠嗅球和视觉皮层的单细胞空间转录组数据.
- * 在CSCP层面应用13种细胞-细胞通信分析方法.
- *分析来自乳腺癌患者的单细胞转录组学数据,以预测免疫疗法反应.
主要成果:
- * 与整体空间分布相比,CSCP内部的单元格具有明显更紧密的空间近距离.
- *CSCP级别的分析揭示了以前在细胞类型水平上隐藏的联体受体相互作用.
- * CSCP 作为有效的预测特征,用于区分抗PD-1 治疗的响应者与乳腺癌的不响应者.
结论:
- *将细胞类型对划分为CSCP,可以更详细地描述细胞与细胞之间的通信.
- *这种方法提高了对正常组织微环境和瘤微环境中信号传递的理解.
- *CSCP为剖析复杂生物系统和预测治疗结果提供了一种新的策略.
更多相关视频
09:34A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
Published on: October 25, 2018
6.7K
08:43A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
11.4K
相关概念视频
Contact-dependent Signaling
44.7K
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
Gap Junctions
In animal cells, gap junctions are formed...
44.7K
Overview of Cell Signaling
20.4K
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
20.4K
Overview of Cell-Matrix Interactions
7.3K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
7.3K
What is Cell Signaling?
119.1K
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
119.1K
Cell Adhesion Molecules - Types and Functions
6.8K
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved...
CAM Families
The Integrin family of proteins is primarily involved...
6.8K
Cytoskeletal Coordination in Cell Migration
4.8K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.8K
